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Anti-Influenza Virus Monoclonal Antibodies

 Influenza viruses are members of the Orthomyxoviridae family, and as such are enveloped viruses that contain segmented genomes composed of negative-sense, single-stranded RNA1). There are three genera of influenza viruses, types A, B and C. Type A influenza viruses are grouped into subtypes based on antigenic properties of the viral-encoded hemagglutinin (HA) and neuraminidase (NA) envelope glycoproteins.
 Monoclonal antibodies are essential tools for many molecular immunology investigations. Especially, anti-influenza virus monoclonal antibodies are useful for diagnosis and investigations of virus structure. These monoclonal antibodies introduced here are suitable for use in ELISA and Western blot.

■Typical Procedure

Figure. Reactivity of the anti-influenza A hemagglutinin monoclonal antibody

 A. ELISA assay. Each well was coated by 50 ng purified influenza hemagglutinin H3. 0-100 ng anti-Influenza A virus Hemagglutinin H3 monoclonal antibody (TCI Product No. : I0779) was added to the well. After washing, bound antibody was detected using goat-anti-mouse IgG-HRP conjugate. The signal was developed with ABTS substrate (TCI Product No. : A2166).
 B. Western Blot. Analysis was performed using anti-Influenza A virus Hemagglutinin H3 monoclonal antibody (I0779) at 1 μg/ml. Goat-anti-mouse-HRP conjugate was used as the secondary antibody. The signal was visualized using DAB-nickel as the chromogen. Molecular weight marker (M). Purified Hemagglutinin (Lane 1).

A2380  Anti-Influenza A Virus Neuraminidase N2 Monoclonal Antibody
(Preservative : 0.05% NaN3, Stabilizer : 1% BSA)

(This product is unavailable in the U.S. and China.)
  Immunogen  :  Influenza A/sydney/5/97(strain H3N2)
  Isotype  :  IgG1
  Host  :  Mouse
  Clone  :  1-4B
  Product Form  :  Protein G purified, mouse monoclonal antibody
  Preservative  :  0.05 % NaN3
  Applications  :  ELISA, Western Blot. Not tested in other applications.

Optimal dilutions are dependent on conditions and should be determined by the user.

A2406  Anti-Influenza A Virus Nucleoprotein Monoclonal Antibody
(Preservative : 0.05% NaN3, Stabilizer : 1% BSA)

(This product is unavailable in the U.S. and China.)
  Immunogen  :  Influenza A/Beijing/262/95
  Isotype  :  IgG2a
  Host  :  Mouse
  Clone  :  17
  Product Form  :  Protein G purified, mouse monoclonal antibody
  Preservative  :  0.05 % NaN3
  Applications  :  ELISA, Western Blot. Not tested in other applications.

Optimal dilutions are dependent on conditions and should be determined by the user.

A2407  Anti-Influenza A Virus Neuraminidase N1 Monoclonal Antibody
(Preservative : 0.05% NaN3, Stabilizer : 1% BSA)

(This product is unavailable in the U.S. and China.)
  Immunogen  :  Influenza A/Beijing/262/95
  Isotype  :  IgG1
  Host  :  Mouse
  Clone  :  2-3B
  Product Form  :  Protein G purified, mouse monoclonal antibody
  Preservative  :  0.05 % NaN3
  Applications  :  ELISA, Western Blot. Not tested in other applications.

Optimal dilutions are dependent on conditions and should be determined by the user.

I0779  Anti-Influenza A Virus Hemagglutinin H3 Monoclonal Antibody
(Preservative : 0.05% NaN3, Stabilizer : 1% BSA)

(This product is unavailable in the U.S. and China.)
  Immunogen  :  purified hemagglutinin H3 (Influenza A/sydney/5/97)5
  Isotype  :  IgG3
  Host  :  Mouse
  Clone  :  1G8
  Product Form  :  Protein G purified, mouse monoclonal antibody
  Preservative  :  0.05 % NaN3
  Applications  :  ELISA, Western Blot. Not tested in other applications.

Optimal dilutions are dependent on conditions and should be determined by the user.

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* These monoclonal antibodies were developed by collaboration with Tokai University.

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