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Animal Tissue-Clearing Reagents"CUBIC"
*CUBIC reagents are commercialized under invention licenses by RIKEN, Japan.
Both of CUBIC-L (Product Number: T3740) and CUBIC-R+ (Product Number: T3741) are required for tissue-clearing.
• Whole-body clearing is achieved using two reagents, CUBIC-L (for delipidation and decoloring) and CUBIC-R+ (for RI matching).
• The quenching of fluorescence signal is low.
• The period of sample treatment is shorter.
• The combination with light-sheet fluorescent microscopy (LSFM) or confocal laser-scanning microscopy (CLSM) enables the whole-organ / body imaging at a cellular resolution.
Example : Mouse whole-body clearing
Figure 1. Whole-body clearing (Left), Whole-body clearing with propidium iodide staining (Right)
1) nuclear staining by propidium iodide (PI)
Example : Mouse whole-organ clearing
Figure 2. Whole-brain clearing (Left),
Whole-brain clearing with RedDot 2 staining and immunostaining (Right)
Example : immunostaining
- Whole-Body Prof iling of Cancer Metastasis with Single-Cell Resolution
- Whole-Brain Imaging with Single-Cell Resolution Using Chemical Cocktails and Computational Analysis
- Whole-Body Imaging with Single-Cell Resolution by Tissue Decolorization