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Biotin-PEG2-NHS (2mg×5)
(CAS RN:596820-83-6 Product Number:B6097)

Structure

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Biotin-PEG2-NHS
Synonym 9-(Biotinamido)-4,7-dioxanonanoic Acid N-Succinimidyl Ester (2mg×5)
Synonym N-Succinimidyl 9-(Biotinamido)-4,7-dioxanonanoate (2mg×5)

General Information

Product Number B6097

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Purity/Analysis Method
Storage Temperature -20°C
M.F. / M.W. C21H32N4O8S=500.57
CAS RN 596820-83-6
Related CAS RN
MDL Number MFCD00467154
Packaging and Container

Notes

Product Information
Volume/Quantity : 2 mg x 5
Molecular weight : 500.6

Description
N-Hydroxysuccinimide (NHS) esters of biotin are commonly used as biotinylation reagents. B6097, Biotin-PEG2-NHS reacts with the primary amines (-NH2) in buffers with pH between 7 and 9. The product is supplied in quantities suitable for biotinylation of up to 40 mg of antibodies.

Storage
This product should be stored at -20 ºC. Do not store at stock solution.

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Directions for Use
Preparation:
Use of a 10 mM Biotin-PEG2-NHS solution is recommended. In order to efficiently biotinylate a sample, Biotin-PEG2-NHS should be used at a 15-fold molar excess over the amount of amine-containing protein. Make sure to calculate the 10 mM biotin solution amount to be used with respect to the molar value of the sample (see example below).
* Do not store reagent in solution.

Calculate A uL of 10 mM biotinylation reagent solution :
For 2mg IgG (150,000 M.W.)
2 [mg IgG] x 10-3 [g/mg] x 1/150,000 [mol/g] x 15 [fold]
= A [uL of 10 mM B6097 solution] x 10-6 [L/uL] x 10 [mmol/L] x 10-3 [mol/mmol]

A = 20 [uL of 10 mM B6097 solution]

Procedure:
1. Bring Biotin-PEG2-NHS (Product No. B6097) to room temperature.
2. Dissolve 2 mg of Biotin-PEG2-NHS (Product No. B6097) in 400uL of PBS and prepare a 10 mM solution of Biotin-PEG2-NHS.
3. Dissolve the sample (1-10 mg/mL) in an amine-free buffer with pH between 7 and 9 such as PBS. Do not use buffers containing amines such as Tris.
4. Add A uL of 10 mM Biotin-PEG2-NHS solution to the sample solution.
5. Incubate the mixed solution for 30 min at room temperature.
6. Remove unreacted and hydrolyzed reagent using desalting column or dialysis methods according to the manufacturer’s instructions.
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Specification

Functionality test to pass test

References

Reaxys-RN 33025816

Application

Biotin-PEG2-NHS (2mg*5)

References

  • D. Savage et al., in Avidin-Biotin Chemistry: A Handbook, 2nd ed., Pierce Chemical Company, 1992, pp. 42-43.

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