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2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
(Product Number:B5834)


2X SDS-PAGE Sample Buffer

General Information

Product Number B5834

* Items available from stock in Japan will be delivered in 10 business days.
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(Sales Dept Tel: +32 (0)3 735 07 00 E-mail: Sales-EU@TCIchemicals.com)
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Product Information
Volume : 25 mL

This product contains 4% SDS, 20% glycerol, 0.001% bromophenol blue and 0.125 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol.
SDS-PAGE Sample Buffer is specially formulated for protein sample preparation to based on the Laemmli Method of SDS-PAGE system.

Description for Use
Preparation of non-reducing sample
A protein sample is mixed with the 2X SDS-PAGE sample buffer (1:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.

Preparation of reducing sample (reducing with 2-Mercaptoethanol)
Add 10 uL of 2-Mercaptoethanol per 90 μL of 2X SDS-PAGE sample buffer. Mix thoroughly. Then a protein sample is mixed with the sample buffer (1:1) and heating to 95–100ºC for 5 min. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
*Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (code: M1948), DL-Dithiothreitol [for Electrophoresis] (code: D3647), etc.

Store at room temperature.

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C3488 Coomassie Brilliant Blue G-250 (Ready-to-use solution) [for Electrophoresis]
D3647 DL-Dithiothreitol [for Electrophoresis]
G0316 Glycerol [for Electrophoresis]
M1948 2-Mercaptoethanol [for Electrophoresis]
S0588 Sodium Dodecyl Sulfate [for Electrophoresis]
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A3217 30% Acrylamide / Bis-acrylamide (29:1) [for Electrophoresis]
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B6104 4X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
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Functionality test Electrophoresis to pass test

Transport Information

HS Number 3822000000


2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]


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