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2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
(Product Number:B5834)

Structure

2X SDS-PAGE Sample Buffer

General Information

Product Number B5834

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Notes

Product Information
Volume : 25 mL

Description
This product contains 4% SDS, 20% glycerol, 0.001% bromophenol blue and 0.125 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol.
SDS-PAGE Sample Buffer is specially formulated for protein sample preparation to based on the Laemmli Method of SDS-PAGE system.

Description for Use
Preparation of non-reducing sample
A protein sample is mixed with the 2X SDS-PAGE sample buffer (1:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.

Preparation of reducing sample (reducing with 2-Mercaptoethanol)
Add 10 uL of 2-Mercaptoethanol per 90 μL of 2X SDS-PAGE sample buffer. Mix thoroughly. Then a protein sample is mixed with the sample buffer (1:1) and heating to 95–100ºC for 5 min. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
*Reducing reagents: 2-Mercaptoethanol [for Electrophoresis] (code: M1948), DL-Dithiothreitol [for Electrophoresis] (code: D3647), etc.

Storage
Store at room temperature.

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C3488 Coomassie Brilliant Blue G-250 (Ready-to-use solution) [for Electrophoresis]
D3647 DL-Dithiothreitol [for Electrophoresis]
G0316 Glycerol [for Electrophoresis]
M1948 2-Mercaptoethanol [for Electrophoresis]
S0588 Sodium Dodecyl Sulfate [for Electrophoresis]
T2516 Tris(hydroxymethyl)aminomethane [for Electrophoresis]
A3217 30% Acrylamide / Bis-acrylamide (29:1) [for Electrophoresis]
A3218 30% Acrylamide / Bis-acrylamide (37.5:1) [for Electrophoresis]
B6104 4X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]
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Specification

Functionality test Electrophoresis to pass test

Transport Information

HS Number 3822000000

Application

2X SDS-PAGE Sample Buffer (2-Mercaptoethanol free) [for Electrophoresis]

References

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