Home >  Products

HPLC Derivatization Reagents

 HPLC is used extensively as a means of detecting and determining trace components. In particular, it is effective in the case of non-volatile trace components in complex matrices such as biological substances. Labeling objective substances for analysis with labeling reagents appropriate for detection methods has been performed to obtain higher sensitivity and selectivity. A great number of labeling reagents have been reported for this purpose.
 NBD- and DBD-derivatives developed by Imai, Toyo’oka and co-workers are excellent fluorescent labeling reagents with strong and long wave-length fluorescence resulting from their benzoxadiazole skeleton and they are used for analysis of various biological substances. NBD-Cl was the earliest fluorescence reagent which was applied to HPLC and its effectiveness for the secondary amines, such as proline, was reported. Imai and co-workers developed NBD-F1), in which the chlorine was replaced with fluorine, for HPLC analysis and obtained good results. Also, they developed DBD-F2) where a dimethylsulfamoyl group was introduced into the benzoxadiazole skeleton and used it for the analysis of amino acids by reversed phase HPLC. Because DBD-F itself is non-fluorescent, DBD-labeled amino acids can be detected and analyzed with high sensitivity. DBD-COCl3) reacts with a hydroxy group as well as amines and thiols, and forms a stable fluorescence adduct. In addition, NBD- and DBD-H4), in which a hydrazino group is introduced into the NBD-, DBD- skeleton, are used for analysis of carbonyl groups. NBD- and DBD-PZ5), in which a piperazino group is introduced, are used for analysis of carboxyl groups.
 For electrochemical detectors (ECD), ferrocene derivatives and NPCA6) have been reported. Ferrocenes have been utilized as the most suitable labeling reagents for ECD because they respond to the application of relatively low voltages, such as +0.5 V, and they are an excellent redox system of oxidation and reduction. NPCA has been developed by the research group of University of Shizuoka recently. NPCA reacts with various primary amines under mild conditions, followed by removal of the THP group to obtain the corresponding α-CA derivatives which have a 6-hydroxychroman skeleton. These derivatives exhibit intense electrochemical activity based on the 6-hydroxychroman skeleton and can be separated efficiently by HPLC on an ODS column. N-(4-Aminobutyl)-N-ethylisoluminol has been reported to be usable as a chemiluminescence labeling reagent.7)

The brochure is in the link below.

UV Detection Labeling Reagents (for Carboxyl Groups) [HPLC Derivatization Reagents]

Page Top

UV Detection Labeling Reagents (for Amino Groups) [HPLC Derivatization Reagents]

Page Top

UV Detection Labeling Reagents (for Hydroxy Groups) [HPLC Derivatization Reagents]

Page Top

UV Detection Labeling Reagents (for Carbonyl Groups) [HPLC Derivatization Reagents]

Page Top

Fluorescence Detection Labeling Reagents (for Carboxy Groups) [HPLC Derivatization Reagents]

A0979
7-Acetoxy-4-bromomethylcoumarin [for HPLC Labeling]
A5502
9-Chloromethylanthracene [for HPLC Labeling]
A5551
Br-Mmc (=4-Bromomethyl-7-methoxycoumarin) [for HPLC Labeling]
A5553
3-Bromomethyl-7-methoxy-1,4-benzoxazin-2-one [for HPLC Labeling]
A5554
NBD-PZ (=4-Nitro-7-piperazino-2,1,3-benzoxadiazole) [for HPLC Labeling]
A5555
DBD-PZ [=4-(N,N-Dimethylaminosulfonyl)-7-piperazino-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5560
(S)-(+)-DBD-APy [=(S)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5561
(R)-(-)-DBD-APy [=(R)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5562
(S)-(+)-NBD-APy [=(S)-(+)-4-Nitro-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5563
(R)-(-)-NBD-APy [=(R)-(-)-4-Nitro-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5570
4-Bromomethyl-6,7-dimethoxycoumarin [for HPLC Labeling]
A5571
DBD-CO-Hz [=4-(N,N-Dimethylaminosulfonyl)-7-(N-hydrazinocarbonylmethyl-N-methyl)amino-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5573
NBD-CO-Hz [=4-(N-Hydrazinocarbonylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5574
DBD-ED [=4-(N,N-Dimethylaminosulfonyl)-7-(2-aminoethylamino)-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5576
AABD-SH (=4-Acetamido-7-mercapto-2,1,3-benzoxadiazole) [for HPLC Labeling]
B1926
9-(Bromomethyl)acridine [for HPLC Labeling]
M0760
7-Amino-4-methylcoumarin [for HPLC Labeling]

Page Top

Fluorescence Detection Labeling Reagents (for Amino Groups) [HPLC Derivatization Reagents]

A1657
(1R,2R)-2-(Anthracene-2,3-dicarboximido)cyclohexanecarboxylic Acid
A1658
(1S,2S)-2-(Anthracene-2,3-dicarboximido)cyclohexanecarboxylic Acid
A5354
Dansylamino-PITC [Fluorescent Coupling Reagent for Edman Degradation]
A5558
DBD-COCl [=4-(N,N-Dimethylaminosulfonyl)-7-(N-chloroformylmethyl-N-methylamino)-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5564
(S)-(-)-DBD-Pro-COCl [=(S)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5565
(R)-(+)-DBD-Pro-COCl [=(R)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5566
(R)-(+)-NBD-Pro-COCl [=(R)-(+)-4-Nitro-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5567
(S)-(-)-NBD-Pro-COCl [=(S)-(-)-4-Nitro-7-(2-chloroformylpyrrolidin-1-yl)-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5568
(R)-(-)-DBD-Py-NCS [=(R)-(-)-4-(N,N-Dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5569
(S)-(+)-DBD-Py-NCS [=(S)-(+)-4-(N,N-Dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5572
NBD-COCl [=4-(N-Chloroformylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole] [for HPLC Labeling]
A5575
DBD-NCS [=4-(N,N-Dimethylaminosulfonyl)-7-isothiocyanato-2,1,3-benzoxadiazole] [for HPLC Labeling and Edman Degradation]
A5577
(R)-(-)-NBD-Py-NCS [=(R)-(-)-4-(3-Isothiocyanatopyrrolidin-1-yl)-7-nitro-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5578
(S)-(+)-NBD-Py-NCS [=(S)-(+)-4-(3-Isothiocyanatopyrrolidin-1-yl)-7-nitro-2,1,3-benzoxadiazole] [HPLC Labeling Reagent for e.e. Determination]
A5579
4-(4,5-Diphenyl-1H-imidazol-2-yl)benzoyl Chloride Hydrochloride [for HPLC Labeling]
A5592
NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]
A5593
NBD-F (=4-Fluoro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]
A5594
2,3-Naphthalenedialdehyde [Fluorimetric Reagent for Primary Amines]
A5595
DBD-F [=4-(N,N-Dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole] [for HPLC Labeling]
D0005
Dansyl Chloride (10% in Acetone)
D0656
Dansyl Chloride
D1669
4-Dimethylamino-1-naphthyl Isothiocyanate [for HPLC Labeling]
D3178
6-[[7-(N,N-Dimethylaminosulfonyl)-2,1,3-benzoxadiazol-4-yl]amino]hexanoic Acid
F0026
Fluorescein 5-Isothiocyanate (isomer I)
F0192
Fluorescamine [for HPLC Labeling]
F0197
9-Fluorenylmethyl Chloroformate [N-Protecting Agent for Peptides Research]
F0783
Fluorescein 6-Isothiocyanate (isomer II)
F0784
Fluorescein Isothiocyanate (mixture of 5- and 6- isomers)
M0722
2-Methoxy-2,4-diphenyl-3(2H)-furanone
M0879
6,7-Methylenedioxy-4-isocyanatomethylcoumarin [for HPLC Labeling]
N0713
(1R,2R)-2-(Naphthalene-2,3-dicarboximido)cyclohexanecarboxylic Acid
N0714
(1S,2S)-2-(Naphthalene-2,3-dicarboximido)cyclohexanecarboxylic Acid
P0280
o-Phthalaldehyde [for HPLC Labeling]
S0503
Succinimidyl 6-[[7-(N,N-Dimethylaminosulfonyl)-2,1,3-benzoxadiazol-4-yl]amino]hexanoate

Page Top

Fluorescence Detection Labeling Reagents (for Hydroxy Groups) [HPLC Derivatization Reagents]

Page Top

Fluorescence Detection Labeling Reagents (for Carbonyl Groups) [HPLC Derivatization Reagents]

Page Top

Fluorescence Detection Labeling Reagents (for Thiol Groups) [HPLC Derivatization Reagents]

Page Top

Chemiluminescence Detection Labeling Reagents [HPLC Derivatization Reagents]

Page Top

Electrochemical Detection Labeling Reagents [HPLC Derivatization Reagents]

Page Top

Page Top