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Bis(2,4,6-trichlorophenyl) Oxalate [Chemiluminescence reagent for the determination of fluorescent compounds]
(CAS RN:1165-91-9 Product Number:A5302)

Structure

Bis(2,4,6-trichlorophenyl) Oxalate
Synonym Oxalic Acid Bis(2,4,6-trichlorophenyl) Ester
Synonym TCPO

General Information

Product Number A5302

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Purity/Analysis Method >98.0%(T)
Storage Temperature
M.F. / M.W. C14H4Cl6O4=448.88
CAS RN 1165-91-9
Related CAS RN
MDL Number MFCD00043061
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Specification

Purity(Argentmetric Titration) min. 98.0 %
Melting point 188.0 to 193.0 deg-C
Solubility in hot Toluene almost transparency
Chemiluminescence to pass test

References

Reaxys-RN 2312135
PubChem SID 87563136

Transport Information

HS Number 2917110090

Application

Chemiluminescence Application in Analytical Chemistry

Chemiluminescence is the phenomenon of luminescence emission when the excited state of a chemical species which occurred by a chemical reaction reverts to the ground state. Bis(2,4,6-trichlorophenyl) Oxalate (TCPO) [A5302], Bis(2,4-dinitrophenyl) Oxalate (DNPO) [A5303] are drawing attention in the aspects of high quantum yields and stability. The emission mechanism is shown below.1)

1) Produce an active intermediate (1,2-dioxetanedione) through the reaction with H2O2.
2) The intermediate excites a fluorescent substance from the ground state to an excited state.
3) The fluorescent substance in the excited state returns to the ground state with the emission of light.

Based on this chemiluminescence reaction, the quantifications of H2O2 and glucose,2) light emitting detection of dansyl amino acid on TLC,3) and quantification of polycyclic aromatic hydrocarbons4) have been reported.

This chemiluminescence reaction was applied to the detection part of HPLC, and 10 fmol level dansyl amino acid was successfully separated and quantified,5) which compared to the conventional method using fluorescence, showed more than 1 order (50 times) higher sensitivity. Furthermore, Imai and co-workers have developed the assay system of dansyl amino acid making use of the DNPO-H2O2 luminous reaction which has been applied to flow-injection analysis.6) The detection limit of dansyl alanine is 5 fmol.

References

PubMed Literature

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