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Animal Tissue-Clearing Reagents"CUBIC"

Tissue-Clearing Reagent
 A tissue clearing method "CUBIC" has been developed by Prof. Hiroki R. Ueda and coworkers at The University of Tokyo / RIKEN. The CUBIC technique enables cyclopedic imaging at a single-cell resolution following whole-body and whole-organ clearing. CUBIC reagents that can be used in the tissue clearing method are provided by TCI.

*CUBIC reagents are commercialized under invention licenses by RIKEN, Japan.

Both of CUBIC-L (Product Number: T3740) and CUBIC-R+ (Product Number: T3741) are required for tissue-clearing.
Please use a combination with CUBIC-HL (Product Number: T3781), CUBIC-B (Product Number: T3780), CUBIC-P (Product Number: T3782) according to your samples.

Advantages

  • Whole-body clearing is achieved using two reagents, CUBIC-L (for delipidation and decoloring) and CUBIC-R+ (for RI matching).

  • These products can easily clear tissues, such as bones or highly fatty tissues, which were previously difficult to clear. (CUBIC-B (for bone), CUBIC-HL (for highly fatty tissues))

  • CUBIC-P for mouse perfusion efficiently aids perfusion fixation.

    Preserve the fluorescent protein signals.

  • The period of sample treatment is shorter.

  • The combination with light-sheet fluorescent microscopy (LSFM) or confocal laser-scanning microscopy (CLSM) enables the whole-organ / body imaging at a cellular resolution.


Example : Mouse whole-body clearing

Whole-body clearing of mouse by CUBIC

Figure 1. Whole-body clearing (Left), Whole-body clearing with propidium iodide staining (Right)

Mouse whole-body clearing procedure
Mouse whole-body clearing procedure
Mouse whole-body clearing procedure for staining
1) nuclear staining by propidium iodide (PI)
Mouse whole-body clearing procedure for nuclear staining by propidium iodide (PI)
2) nuclear staining by RedDot2
Mouse whole-body clearing procedure for nuclear staining by RedDot2

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Example : Mouse whole-organ clearing

Whole-organ clearing of mouse by CUBIC

Figure 2. Whole-brain clearing (Left),
Whole-brain clearing with RedDot 2 staining and immunostaining (Right)

Mouse whole-organ clearing procedure
>Mouse whole-organ clearing procedure
Mouse whole-organ clearing procedure for staining
Example : immunostaining
Mouse whole-organ clearing procedure for staining

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Example : The efficient clearing of adult mouse (more than 6-week-old) whole-body or organ samples

The efficient clearing procedure of adult mouse (more than 6-week-old) whole-body or organ samples
The efficient clearing of adult mouse (more than 6-week-old) whole-body or organ samples

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Example : The clearing of mouse body or tissues including bone

Mouse body or tissues including bone clearing by CUBIC
The clearing procedure of mouse body or tissues including bone
The clearing procedure of Mouse body or tissues including bone

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Example : The clearing of human brain tissue large blocks

The clearing of human brain tissue large blocks by CUBIC
The clearing procedure of human brain tissue large blocks

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Example : The clearing of aggressive human tissue

The clearing procedure of human brain tissue large blocks by CUBIC
The clearing procedure of aggressive human tissue
The clearing procedure of aggressive human tissue

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Brochure with protocols and Q&A

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