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Ethidium Bromide
(CAS RN:1239-45-8 Product Number:E0370)

Structure

Ethidium Bromide
Synonym 3,8-Diamino-5-ethyl-6-phenylphenanthridinium Bromide
Synonym Homidium Bromide

General Information

Product Number E0370

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Purity/Analysis Method >95.0%(HPLC)(T)
Storage Temperature
M.F. / M.W. C21H20BrN3=394.32
CAS RN 1239-45-8
Related CAS RN
MDL Number MFCD00011724
  • Product Details
  • Safety & Regulations
  • Reference & Application

Specification

Appearance Orange to Brown to Dark purple powder to crystal
Purity(HPLC) min. 95.0 area%
Purity(Argentmetric Titration) min. 95.0 %

References

Reaxys-RN 3642536
PubChem SID 87569630
Merck Index(14) 4731
RTECS# SF7950000

GHS

Pictogram
Signal Word Danger
Hazard Statements
  • H302
  • :Harmful if swallowed.
  • H315
  • :Causes skin irritation.
  • H319
  • :Causes serious eye irritation.
  • H330
  • :Fatal if inhaled.
  • H341
  • :Suspected of causing genetic defects.
Precautionary Statements
  • P201
  • :Obtain special instructions before use.
  • P202
  • :Do not handle until all safety precautions have been read and understood.
  • P260
  • :Do not breathe dust, fume, mist, vapors or spray.
  • P264
  • :Wash hands and face thoroughly after handling.
  • P270
  • :Do not eat, drink or smoke when using this product.
  • P271
  • :Use only outdoors or in a well-ventilated area.
  • P280
  • :Wear protective gloves, protective clothing, face protection.
  • P284
  • :Wear respiratory protection.
  • P301+P312+P330
  • :If swallowed: Call a poison center or doctor if you feel unwell. Rinse mouth.
  • P302+P352+P332+P313+P362+P364
  • :If on skin: Wash with plenty of soap and water. If skin irritation occurs: Get medical advice or attention. Take off contaminated clothing and wash it before reuse.
  • P304+P340+P310
  • :If inhaled: Remove person to fresh air and keep comfortable for breathing. Immediately call a poison center or doctor.
  • P305+P351+P338+P337+P313
  • :If in eyes: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice or attention.
  • P308+P313
  • :If exposed or concerned: Get medical advice or attention.
  • P403+P233
  • :Store in a well-ventilated place. Keep container tightly closed.
  • P405
  • :Store locked up.
  • P501
  • :Dispose of contents and container in accordance with local, regional, national regulations (e.g. US: 40 CFR Part 261, EU:91/156/EEC, JP: Waste Disposal and Cleaning Act, etc.).

Transport Information

UN Number 1544
Class 6.1
Packing Group I
HS Number 2933.99.8210

Application

Ethidium Bromide: A Widely Used Dye for DNA and RNA Detection

Ethidium bromide is widely used for DNA and RNA detection. Ethidium bromide binds to double-stranded DNA as an intercalator, inserting itself between the base pairs in the double helix, resulting in changes in the absorption and fluorescence properties of the dye. Ethidium bromide also is used to detect protein–DNA complexes.

References

PubMed Literature

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