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AzBTS (Ready-to-use solution) [for ELISA]
(Numéro de Produit:A3176)

Structure

AzBTS
Synonyme 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic Acid Ammonium Salt) (Ready-to-use solution)

Information générale

Numéro de Produit A3176

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Pureté/Méthode d'analyse
Température de stockage 0-10 °C
F.M. / M.M.
CAS RN
CAS RN concerné
Numéro MDL

Remarques

Product Information
Volume : 100 mL

Description
This product is supplied as a ready-to-use solution containing 2,2'-Azinobis (3-ethylbenzothiazoline-6-sulfonic Acid Ammonium Salt) (AzBTS) and hydrogen peroxide (H2O2) for ELISA. When this product is reacted with horseradish peroxidase (HRP), a green soluble reaction product appears. Its absorbance can be measured at 405 nm.

Direction for Use
The following steps are for a 96-well plate format.
1. Bring AzBTS solution (Product No. A3176) to room temperature.
2. Gently mix the AzBTS solution (Product No. A3176).
3. Add 100uL of AzBTS solution (Product No. A3176) to each well.
4. Incubate the plate at room temperature for 30 minutes.
5. Within 1 hour from start the reaction, measure the absorbance of each well at 405 nm.

Storage
The product should be stored at 4 ºC, and used within 6 months. Don’t freeze because of arising precipitate.

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  • Détails d'un Produit
  • Sécurité et Règlementations
  • Référence & Application

Spécification

Peroxidase detection test Extent of coloring same as reference

SGH

Pictogramme
Terme d'avertissement Warning
Propriétés dangereuses et périlleuses
  • H315
  • :Causes skin irritation.
  • H319
  • :Causes serious eye irritation.
Remarque
  • P264
  • :Wash hands and face thoroughly after handling.
  • P280
  • :Wear protective gloves, eye protection.
  • P302+P352+P332+P313+P362+P364
  • :If on skin: Wash with plenty of soap and water. If skin irritation occurs: Get medical advice or attention. Take off contaminated clothing and wash it before reuse.
  • P305+P351+P338+P337+P313
  • :If in eyes: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice or attention.

Informations de transport

Code Marchandise 3822.00.1090

Application

AzBTS (Ready-to-use solution) [for ELISA]

References

    • Cold Spring Harbor Laboratory Press, New York
      E. Harlow, D. Lane. Antibodies, A Laboratory Manual, 1988, p404, p506, p594.

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