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Streptavidin R-PE Conjugate
(제품번호:T3885)

구조식

Streptavidin R-PE Conjugate
동의어의 SA-R-PE

일반 정보

제품번호 T3885

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순도/분석 방법
보관 온도 0-10 °C
M.F. / M.W.
CAS RN
관련 CAS RN
MDL 번호
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참고

Product Information
Volume/Quantity : 0.1 mg
Product Form : Purified streptavidin, R-Phycoerythrin (R-PE) conjugated.
Conjugation : Purified streptavidin was conjugated to R-PE
(Ex:496 nm, Em:575 nm)
Buffer : Phosphate buffered-saline(-) (PBS(-))
Preservative : 0.05% Sodium azide
Specificity : Biotin

Application
Immunofluorescence (1:200). Flow cytometry (1:1000). FLISA (1:400).
Western blotting (1:1000).

Storage
Store at 4 ºC. Do Not Freeze. This product is photosensitive and should be protected from light.

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  • 제품 정보
  • 안전 및 규정
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규격

Specific Activity to pass test(FLISA)

이용 예

Flow Cytometry of Stained Cells

The HeLa cells were incubated with Mouse Anti CD9 Antibody (red line) or Mouse IgG2aκ isotype control (black line) at 1µg/mL. Then both were incubated with Goat Anti-Mouse IgG Biotin Conjugate [G0387] at 1 µg/mLas a secondary antibody, followed by staining with Streptavidin R-PE Conjugate [T3885] at 1 µg/mL.

References

Immunofluorescence Staining

The HeLa cells were fixed with 4% paraformaldehyde, and permeabilized with 0.1% Triton X-100. Then, the specimen was blocked in BSA/PBS blocking buffer. Subsequently, the specimen was incubated with properly diluted primary antibody (Mouse Anti α-Tubulin IgG and Rabbit Anti AIF* IgG). And, after washing, was further incubated with biotin-conjugated secondary antibody (Goat Anti-Mouse IgG Biotin Conjugate [G0387]). Finally, it was rinsed with PBS and stained with each fluorescent-probe**.
*AIF normally localize in the inner mitochondrial membrane.
**Streptavidin R-PE Conjugate [T3885] at 5 µg/mL (shown in red)
  Goat Anti-Rabbit IgG FITC Conjugate [G0452] at 5 µg/mL (shown in green)
  Hoechst 33258 [H1343] at 1 µg/mL (shown in blue)

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