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Biotin-LC-LC-NHS (2mg×5)
(CAS RN:89889-52-1 產品號碼:B6096)

結構式

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Biotin-LC-LC-NHS
別名 (英文) N-[6-(Biotinamido)hexanoyl]-6-aminohexanoic Acid N-Succinimidyl Ester (2mg×5)
別名 (英文) N-Succinimidyl N-[6-(Biotinamido)hexanoyl]-6-aminohexanoate (2mg×5)

基本信息

產品號碼 B6096

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純度/分析方法
儲藏溫度 -20 °C
分子式 / 分子量 C26H41N5O7S=567.70
CAS RN 89889-52-1
相關CAS RN
MDL號碼 MFCD00467154
包裝

產品說明

Product Information
Volume/Quantity : 2 mg x 5
Molecular weight : 567.7

Description
N-Hydroxysuccinimide (NHS) esters of biotin are commonly used as biotinylation reagents. B6096, Biotin-LC-LC-NHS reacts with the primary amines (-NH2) in buffers with pH between 7 and 9. The product is supplied in quantities suitable for biotinylation of up to 30 mg of antibodies.

Storage
This product should be stored at -20 ºC. Do not store at stock solution.

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Directions for Use
Preparation:
Use of a 10 mM Biotin-LC-LC-NHS solution is recommended. In order to efficiently biotinylate a sample, Biotin-LC-LC-NHS should be used at a 15-fold molar excess over the amount of amine-containing protein. Make sure to calculate the 10 mM biotin solution amount to be used with respect to the molar value of the sample (see example below).
* Do not store reagent in solution.

Calculate A uL of 10 mM biotinylation reagent solution :
For 2mg IgG (150,000 M.W.)
2 [mg IgG] x 10-3 [g/mg] x 1/150,000 [mol/g] x 15 [fold]
= A [uL of 10 mM B6096 solution] x 10-6 [L/uL] x 10 [mmol/L] x 10-3 [mol/mmol]

A = 20 [uL of 10 mM B6096 solution]

Procedure:
1. Bring Biotin-LC-LC-NHS (Product No. B6096) to room temperature.
2. Dissolve 2 mg of Biotin-LC-LC-NHS (Product No. B6096) in 350uL of dimethyl sulfoxide (DMSO) or dimethylformamide (DMF) and prepare a 10 mM solution of Biotin-LC-LC-NHS.
3. Dissolve the sample (1-10 mg/mL) in an amine-free buffer with pH between 7 and 9 such as PBS. Do not use buffers containing amines such as Tris.
4. Add A uL of 10 mM Biotin-LC-LC-NHS solution to the sample solution.
5. Incubate the mixed solution for 30 min at room temperature.
6. Remove unreacted and hydrolyzed reagent using desalting column or dialysis methods according to the manufacturer’s instructions.
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規格

Functionality test to pass test

文獻

Reaxys-RN 8377428

應用

Biotin-LC-LC-NHS (2mg*5)

References

  • D. Savage et al., in Avidin-Biotin Chemistry: A Handbook, 2nd ed., Pierce Chemical Company, 1992, pp. 42-43.

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