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Chemiluminescence Enhancer

In research for life-science, one of the frequently used chemiluminescence (CL) reagents is luminol. Luminol reacts with hydrogen peroxide in the presence of a catalyst, for example, enzymes, metals such as iron, or metal-complexes to emit strong light. In the case of luminol, blue light with a wavelength of 460 nm is emitted.
Since Thorpe et al. reported that phenolic compounds such as 4-iodophenol enhance CL by a peroxidase-luminol-hydrogen peroxide reaction,1) many improved methods have been reported and are called enhanced chemiluminescence (ECL). ECL is one of the sensitive and rapid detection methods in medical and analytical biochemistry.2,3)


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