Br-Mmc (=4-Bromomethyl-7-methoxycoumarin) [for HPLC Labeling]

The compound 1 is an HPLC fluorescence labeling reagent, which has a bromomethyl group, can easily react with a carboxyl group to form the corresponding ester in the presence of a base. The resultant ester is stable enough to reach the detector without any decomposition under reversed phase HPLC. Furthermore, it has a characteristic fluorescence based on a coumarin skeleton, thus an excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 328 nm and 380 nm, respectively.

Application examples:
[Fatty acids] ¹⁾
0.05 g of the labeling reagent 1 and 0.5 g of K₂CO₃ powder is added to a acetone solution (5 mL) of a sample (0.01 g), and incubate at 60 °C for 1 h. After cooling to room temperature, use it as the HPLC sample solution.
[Others]
Carboxylic acids²⁾，aliphatic acids³⁾，dicarboxylic acids⁴⁾，prostagrandins⁵⁾，bile acids⁶⁾，
barbitals⁷⁾

TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )