3-Bromomethyl-7-methoxy-1,4-benzoxazin-2-one [for HPLC Labeling]

The compound 1 is an HPLC fluorescence labeling reagent, which has a bromomethyl group, can easily react with a carboxyl group to form the corresponding ester in the presence of a base. The resultant ester is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 355 nm and 430 nm, respectively.

Application examples:
[Fatty acids] ¹⁾
A solution of the labeling reagent 1 (0.1 mL, 1.0 mM acetonitrile solution) is added to a solution of a fatty acid (0.5 mL, 0.2~10 nmol in acetonitrile). To this solution, a saturated K₂CO₃ / acetonitrile solution (0.5 mL) containing 18-crown 6-ether (5.7 mM) is added, and incubate at 40 °C for 30 min. After cooling to room temperature, use it as the HPLC sample solution.

TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )