AABD-SH (=4-Acetamido-7-mercapto-2,1,3-benzoxadiazole) [for HPLC Labeling]

The compound 1, an HPLC fluorescence labeling reagent, which has a 2,1,3-benzoxadiazole skeleton and a mercapto group, easily reacts with a carboxyl group to form the corresponding thioester. 1 itself fluoresces very little, but the thioester derivatives fluoresce highly. The resultant thioester is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 368 nm and 524 nm, respectively. [The detection limit = 10~20 fmol (S/N = 3)]

Application example:
Add 20 µL of mixed fatty acid / acetonitrile solution, 20 µL of 20 mM labeling reagent 1 / dichloromethane solution, 20 µL of triphenylphosphine / acetonitrile solution and 20 µL of 2,2’-dipyridyl disulfide / acetonitrile solution to a 500 µL vessel, and the mixture is left at room temperature for 15 min. Dilute this reactant mixture with 20 µL of acetonitrile, and use 1 µL of this diluted solution as an HPLC sample solution.

TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )