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CAS RN: 23491-45-4 | Product Number: B6236
Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]
![Thumbnail of Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]](/medias/B6236.jpg?context=bWFzdGVyfHJvb3R8MzkxOHxpbWFnZS9qcGVnfGFHVTJMMmhoWlM4NU1UUXdPRGM0T1RZMk9ERTBMMEkyTWpNMkxtcHdad3w4NDkxNWZiNDE2MWM0MTlkOTYxNGU2OTIyMGYwNTE3ZGRiYTQ5ZDU2ZTJkNjA3ZTFjNTI0Y2YwMmI0ZTg2ZDg2 "Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]")
![Thumbnail of Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]](/medias/Tci-98-B6236-Nuclei-of-HeLa-cells-stained-by-Bisbenzimide-H-33258.jpg?context=bWFzdGVyfHBkcC1hcHBsaWNhdGlvbi1pbWFnZXN8NDg4OXxpbWFnZS9qcGVnfGFHRTFMMmhqWVM4NU5URTBNVE14TnpFNE1UYzBMMVJqYVMwNU9GOUNOakl6TmkxT2RXTnNaV2t0YjJZdFNHVk1ZUzFqWld4c2N5MXpkR0ZwYm1Wa0xXSjVMVUpwYzJKbGJucHBiV2xrWlMxSUxUTXpNalU0TG1wd1p3fGU2ZThlZjdlNjU3Mjg5Yzc5OTljNDk3OGUyN2FkNTE5NzJmZWUyMjJjZDVmNDNjYmVlYmEyNGYwZWU5NjE0ZGQ "Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]")
![Chemical Structure of Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]](/medias/B6236.jpg?context=bWFzdGVyfHJvb3R8MzAxNTZ8aW1hZ2UvanBlZ3xhRGs1TDJnNU5TODVNVFF3TnpjNE1UQTJPVEV3TDBJMk1qTTJMbXB3Wnd8M2VlY2U3ODI5MGI2YWY3N2IwZjdmYTgxOTFjOWQ0MzAxNTYyOTY3ZTI0ZjU4ZTYzY2YwMDdjYzQ4NzdmNmU5OQ "Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]")
![Chemical Structure of Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]](/medias/Tci-440-B6236-Nuclei-of-HeLa-cells-stained-by-Bisbenzimide-H-33258.jpg?context=bWFzdGVyfHBkcC1hcHBsaWNhdGlvbi1pbWFnZXN8NTA4MTl8aW1hZ2UvanBlZ3xhR0UzTDJoak55ODVOVEUwTVRNeE5qVXlOak00TDFSamFTMDBOREJmUWpZeU16WXRUblZqYkdWcExXOW1MVWhsVEdFdFkyVnNiSE10YzNSaGFXNWxaQzFpZVMxQ2FYTmlaVzU2YVcxcFpHVXRTQzB6TXpJMU9DNXFjR2N8Y2Q0NTBiMTIzOTk1ZGVmOGZmMGRmNzUxOGU4ZjI0MDg1ZjZhMmZkNjVkNWZhMGExOTU3ZGM5M2I0MzI0N2M3Mg "Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]")
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![Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]](/_ui/responsive/theme-tci/images/missing_product_zoom.webp "Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]")
Purity:
Synonyms:
- 2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole Trihydrochloride (0.2mL×5) (1mg/mL in Water)
- 4-[5-(4-Methyl-1-piperazinyl)-1H,1'H-[2,5'-bibenzimidazol]-2'-yl]phenol Trihydrochloride (0.2mL×5) (1mg/mL in Water)
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
Shipping Information
|
|---|---|---|---|---|---|
| 1SET |
€30.00
|
3 | 26 |
|
* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)
Supplemental Product Information:
Product Information
- Volume : 0.2 mL x 5 vial
- Concentration : 1.0 mg/mL
Description
- Bisbenzimide H 33258 is a fluorescent stain for labeling DNA.
DNA-bound Bisbenzimide H 33258 is readily excited with UV and emits fluorescence in the blue region of the visible light spectrum (Excitation : 355 nm / Emission : 465 nm).
Direction for Use
- Fluorescence Staining of Cell Nucleus
- Fix and permeabilize cells as desired.
Dilute Bisbenzimide H 33258 solution 1/1000* with PBS or an appropriate buffer. - *Typical working concentration used in most applications is 0.1 μg/mL - 10 μg/mL.
Incubate with cells at room temperature for 5 - 30 minutes** - ** Extend Incubate time as desired.
Mount sample with an appropriate medium and proceed to imaging.
Storage
- Store at −20 °C for long term storage. It may be kept at 4 °C for short term storage. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
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| Product Number | B6236 |
| Molecular Formula / Molecular Weight | C__2__5H__2__4N__6O·3HCl = 533.88 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Frozen (<0°C) |
| Condition to Avoid | Light Sensitive,Heat Sensitive |
| CAS RN | 23491-45-4 |
| Reaxys Registry Number | 4088183 |
| PubChem Substance ID | 468590991 |
| MDL Number | MFCD00012679 |
Specifications
| Appearance | Light yellow to Yellow clear liquid |
| DNA staining test | pass test |
Properties (reference)
| Boiling Point | 100 °C |
GHS
Related Laws:
| EC Number | 245-690-6 |
| RTECS# | SM1140500 |
Transport Information:
| HS Number | 2933599590 |
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