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CAS RN: | Product Number: B6279
Nervous Tissue Protein Extraction Buffer
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Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Philadelphia, PA | Portland, OR | Japan* | Quantity |
|---|---|---|---|---|---|
| 100ML |
$84.00
|
Contact Us | 1 | 7 |
|
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Supplemental Product Information:
Product Information
Volume : 100 mLAdditives : WITHOUT protease inhibitors
Description
This product is supplied as a ready-to-use solution to lyse nervous tissue. Extracted protein can be used in downstream applications such as western blotting and protein quantification. This product does not include protease inhibitors.
Directions for Use
1. Wash nervous tissue twice with PBS.
2. Weigh samples. For each gram of sample, add 10 mL of Nervous Tissue Protein Extraction Buffer (Product No. B6279) and homogenize.
3. Transfer the homogenate to a new tube.
4. Incubate on ice for 10 minutes.
5. Centrifuge the sample at 10,000 × g for 10 minutes.
6. Transfer the supernatant, containing extracted proteins, to a new tube for downstream applications.
Storage
Store at 4 °C and use within 6 months.
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| Product Number | B6279 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Colorless to Almost colorless clear liquid |
| Suitability for protein-analysis | to pass test |
| pH | 7.0 to 8.0 |
Properties (reference)
| Specific Gravity (20/20) | 1.01 |
GHS
Related Laws:
Transport Information:
| HS Number | 3822.19.0080 |
Application
Nervous Tissue Protein Extraction
References
- Quantification of the brain proteome in Alzheimer's disease using multiplexed mass spectrometry
Product Articles
[Featured Products] Ready-to-use Solutions for Protein Extraction[TCIMAIL No.188] Ready-to-use Solution for Protein Extraction from Nervous Tissue
Product Documents (Note: Some products will not have analytical charts available.)
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