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Serotonin and metabolites

Serotonin and metabolites
Column TCI Dual ODS-CX10 (5μm)  4.6 mmI.D. X 150 mm
Mobile Phase A: CH3CN / 50 mmol/L HCOONH4 (pH3.5, HCOOH) = 10/90
B: CH3CN / 50 mmol/L HCOONH4 (pH3.5, HCOOH) = 70/30
  A to 100% B in 60min
Temperature 25 °C
Detection UV 254 nm
Flow Rate 1.0 mL/min
Sample 1.5-Hydroxy-L-tryptophan 2.5-Hydroxyindole-3-acetic Acid 3.N-Acetylserotonin 4.L-Tryptophan 5.Serotonin 6.Melatonin