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CAS RN: 28718-90-3 | Product Number: D5888
DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining]
![Chemical Structure of DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining]](/medias/D5888.jpg?context=bWFzdGVyfHJvb3R8MjkxNzJ8aW1hZ2UvanBlZ3xhREpoTDJneE15ODVNVE13TVRJMU5qZzRPRFl5TDBRMU9EZzRMbXB3Wnd8NTViZjJjMzY3ZmEyMzI5NTkzNTQ3OGMxMjE0YjJiYmYwNTkyMjFmMTAzMDVkMTdkYzllMzVlMWZlMjBkMWUzYg "DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining]")
![Chemical Structure of DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining]](/medias/Tci-440-D5888-Nuclei-of-HeLa-cells-stained-by-DAPI-2HCl.jpg?context=bWFzdGVyfHBkcC1hcHBsaWNhdGlvbi1pbWFnZXN8Nzc4MDN8aW1hZ2UvanBlZ3xhRGc1TDJnM09TODVOVEUwTVRNek1ESTRPRGswTDFSamFTMDBOREJmUkRVNE9EZ3RUblZqYkdWcExXOW1MVWhsVEdFdFkyVnNiSE10YzNSaGFXNWxaQzFpZVMxRVFWQkpMVEpJUTJ3dWFuQm58YzVjYWRjZjU2YjJlNjliM2U4NDAxZmU4NDJiN2ViZDhiYWY2N2EwZjMwYmU1ZjJlOTIzYmFmODkzZDM1NWJjYQ "DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining]")
![Thumbnail of DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining]](/medias/D5888.jpg?context=bWFzdGVyfHJvb3R8NDAxMHxpbWFnZS9qcGVnfGFHRmlMMmhpWXk4NU1UTXdNVE13TURFME1qTTRMMFExT0RnNExtcHdad3xiOGI3MzRiMDE5NmRiMDhhY2I1MjNhOTYwZDNiNGJjZjQ3NDU0NTg0MDY5ZmZiZDQ5OGI3MzYxMDAwM2ExOGEy)
![Thumbnail of DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining]](/medias/Tci-98-D5888-Nuclei-of-HeLa-cells-stained-by-DAPI-2HCl.jpg?context=bWFzdGVyfHBkcC1hcHBsaWNhdGlvbi1pbWFnZXN8NjMwMnxpbWFnZS9qcGVnfGFHTTNMMmczT0M4NU5URTBNVE16TURrME5ETXdMMVJqYVMwNU9GOUVOVGc0T0MxT2RXTnNaV2t0YjJZdFNHVk1ZUzFqWld4c2N5MXpkR0ZwYm1Wa0xXSjVMVVJCVUVrdE1raERiQzVxY0djfGU0MTZjM2U3Y2FkMmE0NTM3MTNhN2JiZTI0N2M3MDQ1NDIzYTI5ZDcwODUzN2FiZDY0NmIwM2RmZTMxNjQ5MTI)
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![DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining]](/_ui/responsive/theme-tci/images/missing_product_zoom.webp "DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining]")
Purity:
Synonyms:
- 4',6-Diamidino-2-phenylindole Dihydrochloride (0.2mL×5) (1mg/mL in Water)
- 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide Dihydrochloride (0.2mL×5) (1mg/mL in Water)
- 6-Amidino-2-(4-amidinophenyl)indole Dihydrochloride (0.2mL×5) (1mg/mL in Water)
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
Shipping Information
|
|---|---|---|---|---|---|
| 1SET |
€38.00
|
2 | 39 |
|
* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)
Supplemental Product Information:
Product Information
- Volume : 0.2 mL x 5 vial
- Concentration : 1.0 mg/mL
Description
- The DAPI is a fluorescent stain for labeling DNA.
DNA-bound DAPI is readily excited with UV light and emits fluorescence in the blue region of the visible light spectrum (Excitation : 358 nm / Emission : 461 nm).
Description for Use
- Fluorescence Staining of Cell Nucleus
- Fix and permeabilize cells as desired.
- Dilute DAPI solution 1/1000 (*) with PBS or an appropriate buffer.
*Typical working concentration used in most applications is 0.1 μg/mL - 10 μg/mL. - Incubate with cells at room temperature for 5-20 minutes (**).
**Extend Incubate time as desired. - Mount sample with an appropriate medium and proceed to imaging.
Storage
- Store at -20 °C for long term storage. It may be kept at 4 °C for short term storage. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
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| Product Number | D5888 |
| Molecular Formula / Molecular Weight | C__1__6H__1__5N__5·2HCl = 350.25 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Frozen (<0°C) |
| Condition to Avoid | Light Sensitive,Heat Sensitive |
| CAS RN | 28718-90-3 |
| Reaxys Registry Number | 4894417 |
| PubChem Substance ID | 468591628 |
| MDL Number | MFCD00012681 |
Specifications
| Appearance | Light yellow to Yellow clear liquid |
| DNA staining test | pass test |
Properties (reference)
| Maximum Absorption Wavelength | 342 nm (H__2O) |
GHS
Related Laws:
| EC Number | 249-186-7 |
| RTECS# | NL5995054 |
Transport Information:
| HS Number | 2933998090 |
Application
Fluorescent staining of DNA in cells
References
- Binding of 4',6-diamidino-2-phenylindole (DAPI) to AT Regions of DNA: Evidence for an Allosteric Conformational Change
- Photoconversion of DAPI and Hoechst dyes to green and red emitting forms after exposure to UV excitation
- Protein kinase C involvement in aloe-emodin- and emodin-induced apoptosis in lung carcinoma cell
- Prostaglandin EP2 receptor downstream of Notch signaling inhibits differentiation of human skeletal muscle progenitors in differentiation conditions
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