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CAS RN: 23491-45-4 | Product Number: H1343

2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole Trihydrochloride Hydrate [for Biochemical Research]

Purity: >98.0%(HPLC)
  • Bisbenzimide H 33258 Hydrate
3   ≥20 

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Product Number H1343
Purity / Analysis Method >98.0%(HPLC)
Molecular Formula / Molecular Weight C__2__5H__2__4N__6O·3HCl·xH__2O = 533.88  
Physical State (20 deg.C) Solid
Storage Temperature <0°C
Condition to Avoid Light Sensitive,Heat Sensitive
CAS RN 23491-45-4
Reaxys Registry Number 4088183
PubChem Substance ID 172089156
MDL Number


Appearance Light yellow to Brown to Dark green powder to crystal
Purity(HPLC) min. 98.0 area%
Water(Vaporization methoed) max. 20.0 %
DNA staining test to pass test
Properties (reference)
Melting Point 280 °C
Pictogram Pictogram
Signal Word Warning
Hazard Statements H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
Precautionary Statements P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ eye protection/ face protection.
P302 + P352 : IF ON SKIN: Wash with plenty of water.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P362 + P364 : Take off contaminated clothing and wash it before reuse.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
Related Laws: / Compliance with laws, Regulations
Enzyme Commission 245-690-6
RTECS# SM1140500
Transport Information:
HS Number 3212900000
Fluorometric Assays of DNA Using Bisbenzimide H 33258

A simple, rapid, and sensitive DNA assay procedure

C. Labarca, K. Paigen, Anal. Biochem. 1980, 102, 344.

Fluorometric assay of DNA in cartilage explants using Hoechst 33258

Y.-J. Kim, R. L. Y. Sah, J.-Y. H. Doong, A. J. Grodzinsky, Anal. Biochem. 1988, 174, 168.

Sequence-Specific Gene Detection with a Gold Electrode Modified with DNA Probes and an Electrochemically Active Dye

K. Hashimoto, K. Ito, Y. Ishimori, Anal. Chem. 1994, 66, 3830.

DNA fluorometric assay in 96-well tissue culture plates using Hoechst 33258 after cell lysis by freezing in distilled water

R. Rage, J. Mitchen, G. Wilding, Anal. Biochem. 1990, 191, 31.

Specific binding of Hoechst 33258 to the d(CGCAAATTTGCG)2 duplex: calorimetric and spectroscopic studies

I. Haq, J. E. Ladbury, B. Z. Chowdhry, T. C. Jenkins, J. B. Chaires, J. Mol. Biol. 1997, 271, 244.

Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study

J. Portugal, M. J. Waring, Biochim. Biophys. Acta 1988, 949, 158.

PubMed Literature

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