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CAS RN: | Product Number: I1275
Clearing Reagent iCBiofilm-D [for Biofilm]
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Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
Shipping Information
|
|---|---|---|---|---|---|
| 8ML |
€120.00
|
Contact Us | 9 |
|
* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)
Supplemental Product Information:
Product Information
- Size : 8 mL
- Sterility : Filter sterilized
Description
- Biofilms are highly organized structures of microbial communities composed of a matrix of polysaccharides and other substances secreted by microbes and/or aggregates of microbial cells. Microbial cells become resistant to antimicrobial drugs and host defense systems when they form biofilms. Clearing Reagent iCBiofilm-D has been developed to allow for live-cell-imaging of biofilm at thicknesses of more than several tens of micrometers. Simple addition of iCBiofilm-D solution to a biofilm renders it transparent within several seconds, allowing for easy observation using light microscopy techniques. This product has been filter-sterilized.
*This product was developed by Associate Prof. Sugimoto.
Storage
- Store at room temperature.
Note
-
- For Research Use Only. Not for Diagnostic or Therapeutic Use.
- Vigorous manipulation of this product may cause biofilm to peel off.
- 24-hour live cell imaging of biofilm can be observed.
- If clear images cannot be obtained or biofilm formation is prevented in iCBiofilm-D, try diluting iCBiofilm-D twice by culture medium.
Directions for Use (for live-cell-imaging of biofilm)
-
- Prepare a live-cell-imaging medium. Dissolve the culture medium powder in iCBiofilm-D to create a biofilm culture medium by autoclaving and so on.
- Prepare a biofilm in a glass bottomed culture dish (35mm diam.) in ordinary medium (not live-cell-imaging medium). Add staining reagents as needed. Culture conditions (including medium, temperature, time, etc.) all play roles in biofilm formation; the conditions specific to your microbe of interest must be determined separately.
- After removing the culture medium, wash the dish with the ordinary medium to remove planktonic cells.
- After wash, add the live-cell-imaging medium. Add staining reagents as needed.
- Observe the biofilm and obtain the images of live-cell-imaging.
Related Products
- T4031 Clearing Reagent iCBiofilm-H1 [for Biofilm]
- T4032 Clearing Reagent iCBiofilm-H2 [for Biofilm]
| Product Number | I1275 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Room Temperature (Recommended in a cool and dark place, <15°C) |
Specifications
| Appearance | Colorless to Light yellow clear liquid |
| Refractive index | 1.3900 to 1.4200 |
| Functionality test | to pass test |
Properties (reference)
GHS
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Transport Information:
| HS Number | 3822190090 |
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Other Documents
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