Maximum quantity allowed is 999
| Size | Unit Price | Philadelphia, PA | Portland, OR | Japan* | Quantity |
|---|---|---|---|---|---|
| 1KIT |
$689.00
|
Contact Us | Contact Us | Contact Us |
|
* Items in stock locally ship in 1-2 business days. Items from Japan stock are able to ship from a US warehouse within 2 weeks. Please contact TCI for lead times on items not in stock. Excludes regulated items and items that ship on ice.
* To send your quote request for bulk quantities, please click on the "Request Quote" button. Please note that we cannot offer bulk quantities for some products.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
This kit contains buffers and typical nuclear stains for 3D nuclear staining of CUBIC-cleared tissues.
Please see the detail protocols in CUBICStars’ web site.
https://www.cubicstars.com/cubic-hv/index.html
Constituents in kit (suitable for a brain of an adult mouse)
Process outline
====Delipidation====
Perfusion fixation and dissection of the mouse brain
Post-fix <24 h
Wash (PBS) 2 h x3
Delipidation (CUBIC-L; Product No. T3740) 3-7 days
Wash (PBS) 2 h x3
====Nuclear staining by CUBIC-HV™1 3D nuclear staining kit (Product No. C3709) ====
Nuclear staining (DAPI or Propidium Iodide or others in CUBIC-HV™1 3D nuclear staining buffer) 3-5 days
Wash (3D nuclear staining wash buffer; 10mM HEPES buffer, pH 7.5) 2 h x3
==== Optional process; Enzyme reaction ====
====Immunostaining by CUBIC-HV™1 3D immunostaining kit (Product No. C3708) ====
Primary antibody (anti-NeuN antibody in C3708 kit can be used): 5 µg/whole brain
Secondary antibody: anti-Fc Fab (out of the kits), 3.75 µg/whole brain
Primary antibody + secondary antibody reaction / Replacement with CUBIC-HV™1 3D immunostaining buffer, 1.5 h
Immunostaining (CUBIC-HV™1 3D immunostaining buffer with CUBIC-HV™1 3D immunostaining additive (if required)), >1 week
Reaction in the same buffer at 4°C, 1 day
Wash (CUBIC-HV™1 3D immunostaining wash buffer), 2 h x3
====Fix and RI matching====
Post-fix (in 1% Formaldehyde Solution), 1 day
Wash (PBS) 2 h
RI matching (CUBIC-R+; Product No. T3740), <3 days
Microscopic observation
Related Products
T3740 Tissue-Clearing Reagent CUBIC-L [for delipidation and decoloring]| Product Number | C3709 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
| Pictogram |
|
| Signal Word | Warning |
| Hazard Statements | H315 : Causes skin irritation. H319 : Causes serious eye irritation. |
| Precautionary Statements | P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ eye protection/ face protection. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P302 + P352 : IF ON SKIN: Wash with plenty of soap and water. P332 + P313 : If skin irritation occurs: Get medical advice/ attention. P362 : Take off contaminated clothing and wash before reuse. |
| HS Number | 3822.19.0080 |
The requested SDS is not available.
Please Contact Us for more information.
A sample C of A for this product is not available at this time.
The requested analytical chart is not available. Sorry for the inconvenience.
