Clearing Reagent iCBiofilm-H2 [for Biofilm]

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Size	Unit Price	Philadelphia, PA	Portland, OR	Japan*	Quantity
8ML	$99.00	2	Contact Us	26

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Product Information

• Size : 8 mL

Description

• Biofilms are highly organized structures of microbial communities composed of a matrix of polysaccharides and other substances secreted by microbes and/or aggregates of microbial cells. Microbial cells become resistant to antimicrobial drugs and host defense systems when they form biofilms.
  In order to observe the structures of biofilms with a thickness of more than several tens of micrometers, Clearing Reagent iCBiofilm-H2 has been developed. iCBioflim-H2 is supplied as a stock solution. Before adding to biofilm, iCBiofilm-H2 has to be diluted with deionized water to appropriate concentration (undiluted iCBiofilm-H2 may work well). By adding iCBiofilm-H2 solution to a biofilm, the biofilm becomes transparent within several seconds and easy-to-observe by light microscopy such as confocal laser scanning microscopy.
  *This product was developed by Associate Prof. Sugimoto, the Jikei University School of Medicine.

Storage

• Stored at room temperature and used within 6 months.

Direction for Use

• 1. Prepare a biofilm in a glass bottomed culture dish (35mm diam.). Culture conditions (including medium, temperature, time, etc.) all play roles in biofilm formation; the conditions specific to your microbe of interest must be determined separately.
  2. After removing planktonic cells and culture medium, add 1% glutaraldehyde solution in PBS and fix at room temperature for 30 minutes. To preserve immunogenicity for immunofluorescence applications, we recommend substituting 4% paraformaldehyde in PBS.
  3. Remove these glutaraldehyde solution and wash three times with PBS or distilled water.
  4. Stain using appropriate fluorescence reagents.
  5. Dilute iCBiofilm-H2 (Product No. T4032) with deionized water to an appropriate concentration.
     Recommendation ratio; iCBiofilm-H2 : water = 2 : 1 or 1 : 1
     If you need the best performance of iCBioflim-H2 for a target biofilm, please consider to examine different concentrations of iCBiofilm-H2.
  6. Remove staining reagents and add diluted or undiluted iCBiofilm-H2 solution (about 500 µL – 1 mL).
  7. Observe the biofilm by optical microscopy.

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