Make sure to sign up for an account today for exclusive coupons and free shipping on orders over $75!
Maximum quantity allowed is 999
Please select the quantity
CAS RN: | Product Number: Y0021
E.coli / Yeast Protein Extraction Buffer
×
Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Philadelphia, PA | Portland, OR | Japan* | Quantity |
|---|---|---|---|---|---|
| 100ML |
$83.00
|
Contact Us | 1 | 16 |
|
* Items in stock locally ship in 1-2 business days. Items from Japan stock are able to ship from a US warehouse within 2 weeks. Please contact TCI for lead times on items not in stock. Excludes regulated items and items that ship on ice.
* To send your quote request for bulk quantities, please click on the "Request Quote" button. Please note that we cannot offer bulk quantities for some products.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
Supplemental Product Information:
Product Information
- Volume : 100 mL
- Additives : WITHOUT protease inhibitors
Description
- This product is supplied as a ready-to-use solution to lyse cultured E. coli / yeast cells. Extracted protein can be used in downstream applications such as Western blotting and protein quantification. This product does not include protease inhibitors.
Storage
- Store at 4 °C.
Directions for Use: Lysis of E. coli cells
-
- Centrifuge E. coli cells at 5000 × g for 10 minutes.
- Remove as much medium as possible from pellet.
- Resuspend pellet in E.coli / Yeast Protein Extraction Buffer (Product No. Y0021). Use 2-4 mL buffer per gram pellet.
- Incubate for 10 minutes at room temperature.
- Centrifuge lysate at 15,000 × g for 10 minutes.
- Transfer the supernatant, containing extracted proteins, to a new tube for downstream applications.
Directions for Use: Lysis of yeast cells
-
- Centrifuge yeast cells at 3000 × g for 10 minutes.
- Remove as much medium as possible from pellet.
- Resuspend pellet in E.coli / Yeast Protein Extraction Buffer (Product No. Y0021). Use 2-4 mL buffer per gram pellet.
- Agitate at room temperature for 10 minutes.
- Centrifuge lysate at 14,000 × g for 10 minutes.
- Transfer the supernatant, containing extracted proteins, to a new tube for downstream applications.
Related Products
- A2215 AEBSF [for Biochemical Research]
- D3789 Disodium Dihydrogen Ethylenediaminetetraacetate Dihydrate [for Biochemical Research]
- E1337 E-64d [for Biochemical Research]
- P2575 Pyrogallol Red (Ready-to-use solution) [for Protein determination]
- P2949 100×Protease Inhibitor Cocktail (EDTA free)
- P2976 100×Protease Inhibitor Cocktail
| Product Number | Y0021 |
| Physical State (20 deg.C) | Liquid |
| Storage Temperature | Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Colorless to Almost colorlessclear liquid |
| Suitability for protein-analysis (E.coli) | to pass test |
| Suitability for protein-analysis (Yeast) | to pass test |
Properties (reference)
| Specific Gravity (20/20) | 1.06 |
GHS
Related Laws:
Transport Information:
| HS Number | 3822.19.0080 |
Application
E.coli / Yeast Protein Extraction
References
- Non-detergent sulphobetaines: a new class of mild solubilization agents for protein purification
Product Articles
[Featured Products] Ready-to-use Solutions for Protein Extraction[TCIMAIL No.188] Ready-to-Use Solution for Protein Extraction from E. coli / Yeast Cells
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
Please select Language.
The requested SDS is not available.
Please Contact Us for more information.
Specifications
C of A & Other Certificates
Please enter Lot Number
Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.
Sample C of A
This is a sample C of A and may not represent a recently manufactured lot of the product.
A sample C of A for this product is not available at this time.
Analytical Charts
Please enter Lot Number
Incorrect Lot Number. Please input only the 4-5 alphanumeric characters before the hyphen.
The requested analytical chart is not available. Sorry for the inconvenience.