E.coli / Yeast Protein Extraction Buffer

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Size	Unit Price	Philadelphia, PA	Portland, OR	Japan*	Quantity	Shipping Information
100ML	$83.00	Ships from Portland the same day*	1	7		Shipping Simulation

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Product Information

• Volume : 100 mL
• Additives : WITHOUT protease inhibitors

Description

• This product is supplied as a ready-to-use solution to lyse cultured E. coli / yeast cells. Extracted protein can be used in downstream applications such as Western blotting and protein quantification. This product does not include protease inhibitors.

Storage

• Store at 4 °C.

Directions for Use: Lysis of E. coli cells

• 1. Centrifuge E. coli cells at 5000 × g for 10 minutes.
  2. Remove as much medium as possible from pellet.
  3. Resuspend pellet in E.coli / Yeast Protein Extraction Buffer (Product No. Y0021). Use 5-10 mL buffer per gram pellet.
  4. Incubate for 10 minutes at room temperature.
  5. Centrifuge lysate at 15,000 × g for 10 minutes.
  6. Transfer the supernatant, containing extracted proteins, to a new tube for downstream applications.

Directions for Use: Lysis of yeast cells

• 1. Centrifuge yeast cells at 3000 × g for 10 minutes.
  2. Remove as much medium as possible from pellet.
  3. Resuspend pellet in E.coli / Yeast Protein Extraction Buffer (Product No. Y0021). Use 2-4 mL buffer per gram pellet.
  4. Agitate at room temperature for 10 minutes.
  5. Centrifuge lysate at 14,000 × g for 10 minutes.
  6. Transfer the supernatant, containing extracted proteins, to a new tube for downstream applications.

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