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Tags, called affinity tags or epitope tags, are polypeptide chains of a few amino acids to a few dozen amino acids that are added to a protein of interest. Even in the absence of antibodies against the protein of interest, anti-tag antibodies can be used to detect the protein of interest.
Anti-tag antibodies corresponding to various epitope tags such as His, GST, DYKDDDDK, etc. are essential for detection, purification, and localization analysis of recombinant proteins. With high specificity and affinity, they can be used in a wide range of applications such as Western blot, immunoprecipitation, immunostaining, and ELISA, improving reproducibility and efficiency of studies. Products conjugated with biotin or HRP are also available, allowing for flexible selection according to the experimental system.
Anti-6xHis Tag Antibodies
Products
Volume/Quantity
- A2957: 0.1 mg/vial
- A3010: 0.05 mg/vial
- A3075: 0.05 mg/vial
Anti-GST Tag Antibodies
Products
Volume/Quantity
- A3175: 0.1 mg/vial
- A3226: 0.05 mg/vial
Detection of GST with A3175
(GST: 0.1 μg/lane)
Electrophoresis sample
Lane M: Molecular weight marker, Lane 2: GST (non-reduced), Lane 3: GST (reduced)
Anti-DYKDDDDK Tag Antibodies
Products
Volume/Quantity
- M3389: 0.1 mg/vial
- M3400: 0.05 mg/vial
- M3712: 0.05 mg/vial
Detection of DYKDDDDK Tag with M3389 or M3400
(human ITGAX fused with DYKDDDDK tag: 0.1 mg/lane)
Electrophoresis sample
Lane M: Molecular weight marker, Lane 1: human ITGAX fused with DYKDDDDK tag (reduced)