Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]

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Synonyms:

• 2'-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bibenzimidazole Trihydrochloride (0.2mL×5) (1mg/mL in Water)
• 4-[5-(4-Methyl-1-piperazinyl)-1H,1'H-[2,5'-bibenzimidazol]-2'-yl]phenol Trihydrochloride (0.2mL×5) (1mg/mL in Water)

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Size	Unit Price	Belgium	Japan*	Quantity	Shipping Information
1SET	€ 38,00	3	28		Shipping Simulation

* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)

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Product Information

• Volume : 0.2 mL x 5 vial
• Concentration : 1.0 mg/mL

Description

• Bisbenzimide H 33258 is a fluorescent stain for labeling DNA.
  DNA-bound Bisbenzimide H 33258 is readily excited with UV and emits fluorescence in the blue region of the visible light spectrum (Excitation : 355 nm / Emission : 465 nm).

Direction for Use

• Fluorescence Staining of Cell Nucleus
• Fix and permeabilize cells as desired.
  Dilute Bisbenzimide H 33258 solution 1/1000* with PBS or an appropriate buffer.
• *Typical working concentration used in most applications is 0.1 μg/mL - 10 μg/mL.
  Incubate with cells at room temperature for 5 - 30 minutes**
• ** Extend Incubate time as desired.
  Mount sample with an appropriate medium and proceed to imaging.

Storage

• Store at −20 °C for long term storage. It may be kept at 4 °C for short term storage. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

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