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CAS RN: 298-93-1 | Product Number: M3297
MTT [for Biochemical Research]
![Chemical Structure of MTT [for Biochemical Research]](/medias/M3297.jpg?context=bWFzdGVyfHJvb3R8NTEzNDJ8aW1hZ2UvanBlZ3xhRFF3TDJoaE5DODRPVEl5TmpNeU1UVTVNall5TDAwek1qazNMbXB3Wnd8ZTQ4MTJmZmE3NmE3NWFmZDExYTk3N2EyNGVkMzZkMzVlMzgzODk4MmMwMDQwODhmMjMzNmI5OTg5NjU2MTVlYg "MTT [for Biochemical Research]")
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![MTT [for Biochemical Research]](/_ui/responsive/theme-tci/images/missing_product_zoom.webp "MTT [for Biochemical Research]")
Purity: >97.0%(HPLC)
Synonyms:
- 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium Bromide
- Thiazolyl Blue Tetrazolium Bromide
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
Shipping Information
|
|---|---|---|---|---|---|
| 200MG |
36,00 €
|
4 | 15 |
|
|
| 1G |
119,00 €
|
1 | ≥40 |
|
* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)
| Product Number | M3297 |
Purity / Analysis Method 
|
>97.0%(HPLC) |
| Molecular Formula / Molecular Weight | C__1__8H__1__6BrN__5S = 414.33 |
| Physical State (20 deg.C) | Solid |
| Storage Temperature
|
Room Temperature (Recommended in a cool and dark place, <15°C) |
| Store Under Inert Gas | Store under inert gas |
| Condition to Avoid | Light Sensitive,Hygroscopic |
| Packaging and Container
|
1G-Glass Bottle with Plastic Insert (View image), 200MG-Glass Bottle with Plastic Insert (View image) |
| CAS RN | 298-93-1 |
| Reaxys Registry Number | 3825277 |
| PubChem Substance ID | 468592393 |
| SDBS (AIST Spectral DB) | 10829 |
| MDL Number | MFCD00011964 |
Specifications
| Appearance | Light yellow to Brown powder to crystal |
| Purity(HPLC) | min. 97.0 area% |
| Purity(Nonaqueous Titration) | min. 98.0 % |
| Functionality test | to pass test (by Abs method) |
| Sensitiveness | Colorimetric Test : Turn Red purple with 35ppm D-Glucose |
| NMR | confirm to structure |
Properties (reference)
| Melting Point | 195 °C(dec.) |
| Maximum Absorption Wavelength | 380 nm (H__2O) |
| Solubility in water | Slightly soluble |
| Degree of solubility in water | 10,000 mg/l |
| Solubility (slightly sol. in) | Ethanol |
GHS
| Pictogram |
|
| Signal Word | Warning |
| Hazard Statements | H315 : Causes skin irritation. H319 : Causes serious eye irritation. H341 : Suspected of causing genetic defects. |
| Precautionary Statements | P501 : Dispose of contents/ container to an approved waste disposal plant. P201 : Obtain special instructions before use. P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection. P308 + P313 : IF exposed or concerned: Get medical advice/ attention. P337 + P313 : If eye irritation persists: Get medical advice/ attention. |
Related Laws:
| EC Number | 206-069-5 |
| RTECS# | XF8060000 |
Transport Information:
| HS Number | 2934100090 |
Application
Use of a tetrazolium/formazan assay (MTT assay) for cell growth assays
MTT, a tetrazolium salt, is widely used tool in cell biology for measuring cell proliferation and cytotoxicity, first reported by T. Mosmann in 1983.1) With mammalian cells, fractionation studies indicate that the reduced pyridine nucleotide cofactor (NADH) in active mitochondria, is responsible for reduction of MTT to a blue color formazan product.2) Therefore, the assay detects living, but not dead cells and the signal generated is dependent on the degree of activation of the cells.3)
References
- 1) Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
- 2) Mechanism of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction
- 3) Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction (a review)
Product Articles
Product Documents (Note: Some products will not have analytical charts available.)
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Specifications
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Other Documents
Related Products
3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium Bromide
| Product # | D0801 |
| CAS RN | 298-93-1 |
| Purity / Analysis Method: | >98.0%(T) |