Published TCIMAIL newest issue No.199
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CAS RN: | Product Number: M3842
Membrane Protein Extraction Kit
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Purity:
Synonyms:
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
Shipping Information
|
|---|---|---|---|---|---|
| 1KIT |
320,00 €
|
Contact Us | 9 |
|
* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)
Supplemental Product Information:
Product Information
- Components : Permeabilization Buffer, Permeabilization Buffer Additive, Solubilization Buffer
- Size : 50 tests
Description
- This product can be used to extract membrane proteins easily from cultured mammalian cells and tissue. Extracted protein can be used in downstream applications such as Western blotting.
Directions for Use
- Dissolve Permeabilization Buffer Additive in Permeabilization Buffer before first use. Aliquot and store at -20 °C.
- Collect cells via centrifugation and wash twice with cold PBS. Use a cell scraper for detachment of adherent cells.
- Resuspend cells in defrosted Permeabilization Buffer with Additive, adding 500 μL for every 5 x 106 cells.
- Incubate at 4 °C for 10 minutes with constant mixing.
- Centrifuge at 16,000 x g for 15 minutes at 4 °C.
- Transfer the supernatant (cytosolic fraction) to a new tube.
- Resuspend the pellet in cold Solubilization Buffer. Use half the amount of buffer used in step 3.
- Incubate at 4 °C for 30 minutes with constant mixing.
- Centrifuge at 16,000 x g for 15 minutes at 4 °C.
- Transfer the supernatant (membrane fraction) to a new tube.
Storage
- Store at 4 °C.
| Product Number | M3842 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
Properties (reference)
GHS
| Pictogram |
|
| Signal Word | Danger |
| Hazard Statements | H301 + H311 + H331 : Toxic if swallowed, in contact with skin or if inhaled. |
| Precautionary Statements | P261 : Avoid breathing mist or vapours. P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ protective clothing. P301 + P310 + P330 : IF SWALLOWED: Immediately call a POISON CENTER/doctor. Rinse mouth. P302 + P352 + P312 : IF ON SKIN: Wash with plenty of water. Call a POISON CENTER/doctor if you feel unwell. P403 + P233 : Store in a well-ventilated place. Keep container tightly closed. |
Related Laws:
Transport Information:
| UN Number | UN3316 |
| Class | 9 |
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