Published TCIMAIL newest issue No.201
Maximum quantity allowed is 999
| Size | Unit Price | Belgium | Japan* | Quantity |
Shipping Information
|
|---|---|---|---|---|---|
| 1VIAL |
€302.00
|
Contact Us | Contact Us |
|
* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)
Description
This product contains 100 µg of recombinant human EGF produced in E. Coli, lyophilized from a 1mg/mL solution containing 1x PBS. Recombinant human EGF comprises a 53 amino acid-length, non-glycosylated polypeptide chain, with a molecular mass of 6.2kDa. Apart from being a potent mitogenic factor, EGF stimulates the proliferation of a wide variety of epithelial cells, and is a common medium additive in both 2D and 3D cell culture.
Specifications
Biological Activity (Balb/c Proliferation Assay) : ED50 < 0.1 ng/mL (> 1 x 107 IU/mL)Amino Acid Sequence
NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
Storage
Store at -80°C. Post-reconstitution, the addition of a carrier protein such as 0.1% BSA is recommended for improved stability. Avoid multiple freeze-thaw cycles.
| Product Number | R0262 |
| Physical State (20 deg.C) | Solid |
Storage Temperature
|
Frozen (-80°C) |
| Condition to Avoid | Heat Sensitive |
| CAS RN | 62229-50-9 |
| Appearance | White to Almost white powder to crystal |
| Biological Activity (Balb/c-3T3 proliferation assay) | ED50 ≤ 0.1ng/mL |
| Endotoxin LevelEndotoxin Level (chromogenic LAL assay) | max. 1.0 EU/µg |
| Mycoplasma Contamination (PCR) | No bands corresponding to mycoplasma were detected |
| Purity (SDS-PAGE/CBB) | min. 95.0 % |
| HS Number | 3504009090 |
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