Professor Chris Meier

Prof. Meier and TCI cooperate in synthesis and supply of 5-Chloro-saligenyl-N,N-diisopropylphosphoramidite, [C3326], and Bis(Tetra-n-Butylammonium) Dihydrogen Pyrophosphate, [B5440], reagents for triphosphorylation of DNA and RNA snips. The mentioned material belongs to the group of phosphoramidites an inevitable group of starting materials for synthesis of oligonucleotides.

For successful synthesis, the use of booth starting materials, [C3326] and [B5440] are recommend.

The materials are easy to use with all equipment for synthesis of “Oligos”.

Triphosphorylated “Oligos” can be use for:

• Ligation of PCR products into a vector
• Activation of RIG-I protein
• Synthesis of m7G-capped triphosphorylated RNA
• Substrate DNA repair studies
• Permits labelling
• Further chemical modification

For more information please refer:

5’-Triphosphorylated (5’-ppp-)oligonucleotides

Efficient Automated Solid-Phase Synthesis of DNA and RNA 5’-Triphosphates

For successful synthesis follow the protocol below:

Solid-Phase Synthesis of DNA and RNA 5′-O-Triphosphates Using cycloSal Chemistry