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CAS RN: 189373-41-9 | 产品编码: A5574

DBD-ED [=4-(N,N-Dimethylaminosulfonyl)-7-(2-aminoethylamino)-2,1,3-benzoxadiazole] [for HPLC Labeling]


纯度/分析方法:
别名:
  • DBD-ED [=4-(N,N-二甲氨基磺酰)-7-(2-氨基乙基氨基)-2,1,3-苯并恶二唑] [HPLC标记用]
  • 4-(N,N-二甲氨基磺酰)-7-(2-乙二胺基)-2,1,3-苯并恶二唑[用于高效液相色谱标记]
  • 4-(N,N-Dimethylaminosulfonyl)-7-(2-aminoethylamino)-2,1,3-benzoxadiazole
文档:
100MG
¥1,950.00
1   1   ≥20 
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产品编码 A5574
分子式/分子量 C__1__0H__1__5N__5O__3S = 285.32  
外观与形状(20°C) 固体
CAS RN 189373-41-9
Reaxys-RN 18820975
PubChem物质ID 87563191
MDL编号

MFCD01321129

技术规格
Appearance Light yellow to Brown powder to crystal
Elemental analysis(Nitrogen) 23.30 to 25.80 %
Effect test of HPLC derivatization Effective as labeling agent for Linoleic Acid , Oleic Acid
物性(参考值)
GHS
相关法规
物流信息
监管条件代码(*)
应用
HPLC Labeling Reagent for Carboxylic Acids

The compound 1, an HPLC fluorescence labeling reagent, which has a 2,1,3-benzoxadiazole skeleton and an amino group, easily reacts with a carboxyl group to form the corresponding amide in the presence of a condensing agent. The resultant amide is stable enough to reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by fluorescence detection at the excitation and emission wavelengths of 450 nm and 560 nm, respectively. Since their excitation and fluorescence wavelengths are at longer wavelengths, detection has less interference from contaminants. Short-chain fatty acids are detectable and determinable reproducibly with a detection limit on the order of fmol. A highly sensitive detection can be done by using chemiluminescence.

Application example:2)
Add 50 µL of mixed fatty acid / diethyl ether solution, 50 µL of 50 mM labeling reagent 1 / acetonitrile solution, 50 µL of triphenylphosphine / acetonitrile solution and 50 µL of 2,2’-dipyridyl disulfide / acetonitrile solution to a vessel. This mixture is kept in the dark at room temperature. Dilute this reactant mixture 100x by acetonitrile, and use 10 µL of this diluted solution as an HPLC sample solution.

References


参考文献


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