Maximum quantity allowed is 999
CAS RN: | 产品编码: C3709
CUBIC-HV™1 3D nuclear staining kit
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补充产品信息:
This kit contains buffers and typical nuclear stains for 3D nuclear staining of CUBIC-cleared tissues.
Please see the detail protocols in CUBICStars’ web site.
https://www.cubicstars.com/cubic-hv/index.html
Constituents in kit (suitable for a brain of an adult mouse)
- CUBIC-HV™1 3D nuclear staining buffer (1x) (for 10 tests)
- CUBIC-HV™1 3D nuclear staining washing buffer (100x) (for 10 tests)
- DAPI·2HCl (200x) (for 10 tests)
- Propidium Iodide (100x) (for 10 tests)
- 10 x 5 mL tube
Process outline
====Delipidation====
Perfusion fixation and dissection of the mouse brain
Post-fix <24 h
Wash (PBS) 2 h x3
Delipidation (CUBIC-L; Product No. T3740) 3-7 days
Wash (PBS) 2 h x3
====Nuclear staining by CUBIC-HV™1 3D nuclear staining kit (Product No. C3709) ====
Nuclear staining (DAPI or Propidium Iodide or others in CUBIC-HV™1 3D nuclear staining buffer) 3-5 days
Wash (3D nuclear staining wash buffer; 10mM HEPES buffer, pH 7.5) 2 h x3
==== Optional process; Enzyme reaction ====
====Immunostaining by CUBIC-HV™1 3D immunostaining kit (Product No. C3708) ====
Primary antibody (anti-NeuN antibody in C3708 kit can be used): 5 µg/whole brain
Secondary antibody: anti-Fc Fab (out of the kits), 3.75 µg/whole brain
Primary antibody + secondary antibody reaction / Replacement with CUBIC-HV™1 3D immunostaining buffer, 1.5 h
Immunostaining (CUBIC-HV™1 3D immunostaining buffer with CUBIC-HV™1 3D immunostaining additive (if required)), >1 week
Reaction in the same buffer at 4°C, 1 day
Wash (CUBIC-HV™1 3D immunostaining wash buffer), 2 h x3
====Fix and RI matching====
Post-fix (in 1% Formaldehyde Solution), 1 day
Wash (PBS) 2 h
RI matching (CUBIC-R+; Product No. T3740), <3 days
Microscopic observation
Related Products
T3740 Tissue-Clearing Reagent CUBIC-L [for delipidation and decoloring]T3741 Tissue-Clearing Reagent CUBIC-R+ [for RI matching]
C3708 CUBIC-HV™1 3D immunostaining kit
F0622 37% Formaldehyde Solution
H0396 HEPES
产品编码 | C3709 |
外观与形状(20°C) | 液体 |
储存温度 ![]() |
冷藏 (0-10°C) |
应避免的情况 | 加热 |
象形图 |
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信号词 | 警告 |
危险性说明 | H315 : 造成皮肤刺激。 H319 : 造成严重眼刺激。 |
防范说明 | P264 : 作业后彻底清洗皮肤。 P280 : 戴防护手套/戴防护眼罩/戴防护面具。 P302 + P352 : 如皮肤沾染:用水充分清洗。 P337 + P313 : 如仍觉眼刺激:求医/就诊。 P305 + P351 + P338 : 如进入眼睛:用水小心冲洗几分钟。如戴隐形眼镜并可方便地取出,取出隐形眼镜。继续冲洗。 P362+P364 : 脱掉沾污的衣服,清洗后方可重新使用。 P332 + P313 : 如发生皮肤刺激:求医/就诊。 |
监管条件代码(*) |
References
- Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues
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