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Published TCIMAIL newest issue No.201
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CAS RN: 28718-90-3 | Product Number: A2412
DAPI 2HCl [for Biochemical Research]
![Thumbnail of DAPI 2HCl [for Biochemical Research]](/medias/A2412.jpg?context=bWFzdGVyfHJvb3R8MTM4MDh8aW1hZ2UvanBlZ3xhRGxrTDJnMk5DODRPVE16TVRBME56UTJOVEkyTDBFeU5ERXlMbXB3Wnd8YmUyMWQyMWFmZDVjMTVmYWU3YzAxZGU4MzQ0M2I5N2I0MDEwOThkZWM0Njg2MTJjNGM0NWM1Mzc2ZTM0ZjNmZg "DAPI 2HCl [for Biochemical Research]")
![Thumbnail of DAPI 2HCl [for Biochemical Research]](/medias/Tci-98-A2412-DAPI-2HCl-was-used-to-stain-the-nuclei.jpg?context=bWFzdGVyfHBkcC1hcHBsaWNhdGlvbi1pbWFnZXN8MzA5MnxpbWFnZS9qcGVnfGFHUTRMMmhpWkM4NU5URTBNVE14TXprd05EazBMMVJqYVMwNU9GOUJNalF4TWkxRVFWQkpMVEpJUTJ3dGQyRnpMWFZ6WldRdGRHOHRjM1JoYVc0dGRHaGxMVzUxWTJ4bGFTNXFjR2N8MDc2NDFiMWYzZjk5ODljMTViZWMyNDE5YWQ2ZTg2OWNjNzI1ZTE5NDVmMzBlOWMyYzAxMmQ0YTBlODE0M2Q1Zg "DAPI 2HCl [for Biochemical Research]")
![Chemical Structure of DAPI 2HCl [for Biochemical Research]](/medias/A2412.jpg?context=bWFzdGVyfHJvb3R8NDMyNzR8aW1hZ2UvanBlZ3xhREZrTDJoaE5pODRPVEU1TmpZeU56SXpNVEF5TDBFeU5ERXlMbXB3Wnd8ZTIwNjk2NjdhZjZlZTQyY2M1OGY0NTM1MjUyNTU1OWU5ZDRhYzlkODc4YTczMmJkZmU4YjA0ZjY1ZGViMGI4Mg "DAPI 2HCl [for Biochemical Research]")
![Chemical Structure of DAPI 2HCl [for Biochemical Research]](/medias/Tci-440-A2412-DAPI-2HCl-was-used-to-stain-the-nuclei.jpg?context=bWFzdGVyfHBkcC1hcHBsaWNhdGlvbi1pbWFnZXN8NTQyNDJ8aW1hZ2UvanBlZ3xhREUzTDJoaVpDODVOVEUwTVRNeE16STBPVFU0TDFSamFTMDBOREJmUVRJME1USXRSRUZRU1MweVNFTnNMWGRoY3kxMWMyVmtMWFJ2TFhOMFlXbHVMWFJvWlMxdWRXTnNaV2t1YW5CbnxkM2Q0MWFlNDBjOWI2NDQ2MTk1ZDAyMTM5ZTI5NjE3Y2I0N2NhNzYzY2M4MzlmZmM0NzAyMTc0MDk4MGVhYjA3 "DAPI 2HCl [for Biochemical Research]")
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![DAPI 2HCl [for Biochemical Research]](/_ui/responsive/theme-tci/images/missing_product_zoom.webp "DAPI 2HCl [for Biochemical Research]")
Purity: >90.0%(HPLC)
Synonyms:
- 4',6-Diamidino-2-phenylindole Dihydrochloride
- 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide Dihydrochloride
- 6-Amidino-2-(4-amidinophenyl)indole Dihydrochloride
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
Shipping Information
|
|---|---|---|---|---|---|
| 5MG |
45,00 €
|
7 | ≥100 |
|
* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)
| Product Number | A2412 |
Purity / Analysis Method 
|
>90.0%(HPLC) |
| Molecular Formula / Molecular Weight | C__1__6H__1__5N__5·2HCl = 350.25 |
| Physical State (20 deg.C) | Solid |
| Storage Temperature
|
Frozen (<0°C) |
| Condition to Avoid | Light Sensitive,Heat Sensitive |
| CAS RN | 28718-90-3 |
| Reaxys Registry Number | 4894417 |
| PubChem Substance ID | 135727109 |
| MDL Number | MFCD00012681 |
Specifications
| Appearance | Light yellow to Yellow to Orange powder to crystal |
| Purity(HPLC) | min. 90.0 area% |
| DNA staining test | to pass test |
Properties (reference)
GHS
Related Laws:
| EC Number | 249-186-7 |
Transport Information:
| HS Number | 2933998090 |
Application
Fluorescence Staining of Cell Nucleus
DAPI is an organic dye which binds strongly to DNA. DAPI emits a blue fluorescence (Maximum fluorescent wavelength; 461 nm) by the irradiation of UV light (Maximum excitation wavelength; 358 nm).
HeLa cell nucleus stained with DAPI·2HCl [A2412] has been observed by fluorescent microscopy.
HeLa cell nucleus stained with DAPI·2HCl [A2412] has been observed by fluorescent microscopy.
HeLa cells were fixed with 4% paraformaldehyde, and permerabilized with 0.2% Triton X-100.
HeLa cells nuclei were stained with 1 µg/mL DAPI [A2412] solution.
HeLa cells nuclei were stained with 1 µg/mL DAPI [A2412] solution.
References
- Labeling Nuclear DNA Using DAPI
PubMed Literature
Documents
Product Articles
Product Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
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Specifications
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Other Documents
Related Products
![DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining]](/medias/D5888.jpg?context=bWFzdGVyfHJvb3R8MTY2NjB8aW1hZ2UvanBlZ3xhR1ppTDJoaU1pODVNVE13TVRJNE5UY3lORFEyTDBRMU9EZzRMbXB3Wnd8MGZiN2ExODYxMDJkZjEwZWE4YWRlMjc4ZGNmNmRjMTlkYzYyOTkxN2Q4ZGU1YjlmZTY3YTdlNjNmYWQzODA2Mw "DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining]")
DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining]
| Product # | D5888 |
| CAS RN | 28718-90-3 |
| Purity / Analysis Method: |