Published TCIMAIL newest issue No.201
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CAS RN: | Product Number: T3981
Thiol Quantification Kit
×
Purity:
Synonyms:
- Sulfhydryl Quantification Kit
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
Shipping Information
|
|---|---|---|---|---|---|
| 1KIT |
€ 410,00
|
Contact Us | 13 |
|
* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)
Supplemental Product Information:
Product Information
Components : 2 vials of DTNB, 2 vials of N-Acetyl-L-cysteine Standard, Diluent BufferSize : 100 tests
Description
This kit consists of three components; 5,5'-Dithiobis(2-nitrobenzoic Acid) (DTNB, Ellman’s reagent), N-Acetyl-L-cysteine (NAC), and Diluent Buffer. Reaction of DTNB with SH groups causes the solution containing them to turn yellow in color. Use of an N-Acetyl-L-cysteine standard curve allows for the quantification of SH group concentration via absorbance measurement at 412 nm.
Storage
Store at 4 °C. Use within 6 months.
Direction for Use
- Prepare an SH group-containing sample, using Diluent Buffer to increase the volume to 200 µL if necessary.
- Add 1 mL of Diluent Buffer to 1 vial of N-Acetyl-L-cysteine to prepare a 10 mM NAC standard solution. Dissolve carefully by vortexing.
- Preparation of an NAC dilution series:
Dilute the 10 mM NAC standard solution prepared in step 2 10-fold with Diluent Buffer to make a 1000 µM NAC solution (of greater than 400 µL); further dilute this solution two-fold six additional times using Diluent Buffer such that you obtain seven samples (each greater than 200 µL in volume). The dilution series comprises these seven samples, along with a blank containing only Diluent Buffer. The concentration range this dilution series offers is appropriate in many cases, however if you plan on measuring a more dilute sample, adjust concentrations as needed. - Aliquot 100 µL of each sample or NAC solution to each of two wells of a 96-well plate.
- Prepare DTNB solution by adding 1 mL of Diluent Buffer to 1 vial of DTNB. Dissolve carefully by vortexing. (Use DTNB solution as quickly as possible following preparation.)
- Add 15 µL of this DTNB solution to each well.
- Incubate at room temperature for 10 minutes.
- Measure the absorbance at 412 nm. (Broad range measurement from 380 - 440 nm is also possible.)
- Create a standard curve by plotting the absorbance of each standard solution against its SH concentration in μM. Use this standard curve to determine the SH concentration of your samples.
Related Products
D0944 5,5'-Dithiobis(2-nitrobenzoic Acid) [for Determination of SH groups]
| Product Number | T3981 |
Storage Temperature 
|
Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
Properties (reference)
GHS
| Pictogram |
|
| Signal Word | Warning |
| Hazard Statements | H315 : Causes skin irritation. H319 : Causes serious eye irritation. H371 : May cause damage to organs. |
| Precautionary Statements | P501 : Dispose of contents/ container to an approved waste disposal plant. P260 : Do not breathe dust. P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ eye protection/ face protection. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P308 + P311 : IF exposed or concerned: Call a POISON CENTER/doctor. |
Related Laws:
Transport Information:
| HS Number | 3822190090 |
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