Influenza viruses are members of the Orthomyxoviridae family, and as such are enveloped viruses that contain segmented genomes composed of negative-sense, single-stranded RNA.1) There are three genera of influenza viruses, types A, B and C. Type A influenza viruses are grouped into subtypes based on antigenic properties of the viral-encoded hemagglutinin (HA) and neuraminidase (NA) envelope glycoproteins.
Monoclonal antibodies are essential tools for many molecular immunology investigations. Especially, anti-influenza virus monoclonal antibodies are useful for diagnosis and investigations of virus structure. These monoclonal antibodies introduced here are suitable for use in ELISA and Western blot.
Typical Procedure
Figure. Reactivity of the anti-influenza A hemagglutinin monoclonal antibody
A. ELISA assay. Each well was coated by 50 ng purified influenza hemagglutinin H3. 0-100 ng anti-Influenza A virus Hemagglutinin H3 monoclonal antibody (TCI Product No. I0779) was added to the well. After washing, bound antibody was detected using goat-anti-mouse IgG-HRP conjugate. The signal was developed with ABTS substrate (TCI Product No. A2166).
B. Western Blot. Analysis was performed using anti-Influenza A virus Hemagglutinin H3 monoclonal antibody (I0779) at 1 μg/ml. Goat anti-mouse-antibody HRP conjugate was used as the secondary antibody. The signal was visualized using DAB-nickel as the chromogen. Molecular weight marker (M). Purified Hemagglutinin (Lane 1).
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Products
- A2380
- Anti-Influenza A Virus Neuraminidase N2 Monoclonal Antibody (Preservative : 0.05% NaN3, Stabilizer : 1% BSA) (This product is unavailable in the U.S. and China.)
Immunogen : Influenza A/sydney/5/97
Isotype : IgG1
Host : Mouse
Clone : 1-4B
Product Form : Protein G purified, mouse monoclonal antibody, 0.05% NaN3, 1% BSA
Applications : ELISA, Western Blot. Not tested in other applications.
- A2406
- Anti-Influenza A Virus Nucleoprotein Monoclonal Antibody (Preservative : 0.05% NaN3, Stabilizer : 1% BSA) (This product is unavailable in the U.S. and China.)
Immunogen : Influenza A/Beijing/262/95
Isotype : IgG2a
Host : Mouse
Clone : 17
Product Form : Protein G purified, mouse monoclonal antibody, 0.05% NaN3, 1% BSA
Applications : ELISA, Western Blot. Not tested in other applications.
- A2407
- Anti-Influenza A Virus Neuraminidase N1 Monoclonal Antibody (Preservative : 0.05% NaN3, Stabilizer : 1% BSA) (This product is unavailable in the U.S. and China.)
Immunogen : Influenza A/Beijing/262/95
Isotype : IgG1
Host : Mouse
Clone : 2-3B
Product Form : Protein G purified, mouse monoclonal antibody, 0.05% NaN3, 1% BSA
Applications : Western Blot. Not tested in other applications.
- I0779
- Anti-Influenza A Virus Hemagglutinin H3 Monoclonal Antibody (Preservative : 0.05% NaN3, Stabilizer : 1% BSA) (This product is unavailable in the U.S. and China.)
Immunogen : purified hemagglutinin H3 (Influenza A/Sydney/5/97)
Isotype : IgG3
Host : Mouse
Clone : 1G8
Product Form : Protein G purified, mouse monoclonal antibody, 0.05% NaN3, 1% BSA
Applications : ELISA, Western Blot. Not tested in other applications.
* These monoclonal antibodies were developed by collaboration with Tokai University.
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References
- 1) P. F. Wright, R. G. Webster, Orthomyxoviruses, in Fields Virology, 4th ed., Lippincott Williams & Wilkins, Philadelphia, 2001, vol. 1.
- 2) Details of influenza A sydney/5/97
- a) Phenotypic properties resulting from directed gene segment reassortment between wild-type A/Sydney/5/97 influenza virus and the live attenuated vaccine strain
C. L. Parks, T. Latham, A. Cahill, R. E. O'Neill, C. J. Passarotti, D. A. Buonagurio, T. M. Bechert, G. A. D'Arco, G. Neumann, J. DeStefano, H. E. Arendt, J. Obregon, L. Shutyak, S. Hamm, M. S. Sidhu, T. J. Zamb, S. A. Udem, Virology 2007, 367, 275.
- b) Accumulation of amino acid substitutions promotes irreversible structural changes in the hemagglutinin of human influenza AH3 virus during evolution
K. Nakajima, E. Nobusawa, A. Nagy, S. Nakajima, J. Virol. 2005, 79, 6472. - c) Influenza research database
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