N-Chloromethyl-4-nitrophthalimide [for HPLC Labeling]

The compound 1 is an HPLC labeling reagent, which has a chloromethyl group and easily reacts with a carboxyl group to form the corresponding ester in the presence of a base. The resultant ester is stable and can reach the detector without any decomposition under reversed phase HPLC. An excellent chromatogram can be obtained by measuring at 230 nm.

Application example:
[Fatty acids]^{1, 2)}
Dissolve 3 mg of a sample in 1 mL of acetonitrile, and add 1 mL of the labeling reagent 1 / acetonitrile solution (11 mg / mL) and 1 mL of triethylamine / acetonitrile solution (5 mg / mL). Close the cap of the reaction vessel and incubate the solution at 60 °C for 1 h. Cool the resultant solution to room temperature and use it as an HPLC sample. In the case of using alkali metal salts and crown ethers, the esterification reaction is completed in 15 min at 60 °C. Cool the resultant solution to room temperature and use it as an HPLC sample.

TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )