NBD-Cl (=4-Chloro-7-nitro-2,1,3-benzoxadiazole) [for HPLC Labeling]

The compound 1 which is an HPLC fluorescence labeling reagent having a 2,1,3-benzoxadiazole skeleton, can easily react with a secondary amine and thiol. The　resultant derivative is stable enough to reach the detector without any decomposition　under general reversed phase HPLC. An excellent chromatogram can be obtained by　fluorescence detection analysis at the excitation and emission wavelengths of 460 nm and　535 nm, respectively.

Application example:
[Alkylamines] ¹⁾
To 25~500 µL of a methanol solution containing an amine (1~20 µg), 4~8 eq. excess amount of 0.05% labeling reagent 1 / methanol solution is added. After adding 50~100 µL of 0.1 M NaHCO₃, incubate at 55 °C for 1~5 h. After cooling the reaction mixture to room temperature, use it as an HPLC sample.
[Others]
TLC and HPLC of N-methylcarbamates, N,N-dimethylcarbamates in agrichemicals^2,3) Hydrolyze the carbamates to label the amine derivatives.
TLC of amphetamines in urine^4,5), HPLC of prolines (precolumn derivatization method)⁶⁾

TCI Products Pamphlet
HPLC Labeling Reagents ( PDF 10MB )