Coumarin Tyramide 351 [for Tyramide Signal Amplification] (50 tests)

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Reinheit:

Synonyme

• 7-Hydroxy-N-(4-hydroxyphenethyl)-2-oxo-2H-chromene-3-carboxamide (50 tests)

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SDS | Spezifikation | AZ & andere Zertifikate | Analytische Diagramme

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Einheit	Stückpreis	Belgien	Japan*	Menge	Versandinformationen
1VIAL	€129.00	Kontaktieren Sie uns	28		Versandsimulation

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Step-by-Step Protocol

1. Incubate tissue sections as normal, first with primary antibody, followed by HRP-conjugated secondary antibody. (Preliminary testing of primary antibody concentrations between 1:100 and 1:2000 is recommended.)
2. Before first use, dissolve a tyramide reagent in 50 µL DMSO. (Aliquot as needed. Store below -20°C. Minimize freeze-thaw cycles.)
3. After secondary antibody staining, wash sections with TBST (5 minutes, 3 times).
4. Immediately before use, mix the following to prepare the staining solution for each 1 cm² area to be stained.
   • Tyramide reagent solution (from Step 2) 1 µL
   • 0.15% H₂O₂ 1 µL
   • 1x TBS 98 µL
5. Remove slides from TBST and tilt onto a tissue to remove excess buffer. Lay slides flat, immediately apply 100 µL of the staining solution from Step 4 to each section, and allow to react for 1-10 minutes.
6. After the reaction, remove excess solution with a tissue and immediately wash with an excess of TBST (5 minutes, 3 times).
7. After washing, apply mounting solution and coverslip for observation.