Published TCIMAIL newest issue No.201
Maximum quantity allowed is 999
CAS RN: | Product Number: A3176
AzBTS (Ready-to-use solution) [for ELISA]
Purity:
- 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic Acid Ammonium Salt) (Ready-to-use solution)
* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)
Supplemental Product Information:
Product Information
Volume : 100 mLDescription
This product is supplied as a ready-to-use solution containing 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic Acid Ammonium Salt) (= AzBTS) and hydrogen peroxide (H2O2) for ELISA. When this product is reacted with horseradish peroxidase (HRP), a green soluble reaction product appears. Its absorbance can be measured at 405 nm.
Direction for Use
The following steps are for a 96-well plate format.
- Bring AzBTS solution (Product No. A3176) to room temperature.
- Gently mix the AzBTS solution (Product No. A3176).
- Add 100μL of AzBTS solution (Product No. A3176) to each well.
- Incubate the plate at room temperature for 30 minutes.
- Within 1 hour from start the reaction, measure the absorbance of each well at 405 nm.
Storage
The product should be stored at 4 °C, and used within 6 months. Don’t freeze because of arising precipitate.
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| Product Number | A3176 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature
|
Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
| Peroxidase detection test | Extent of coloring same as reference |
| Pictogram |
|
| Signal Word | Danger |
| Hazard Statements | H315 : Causes skin irritation. H319 : Causes serious eye irritation. H372 : Causes damage to organs through prolonged or repeated exposure. H370 : Causes damage to organs. |
| Precautionary Statements | P501 : Dispose of contents/ container to an approved waste disposal plant. P260 : Do not breathe mist or vapours. P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ eye protection/ face protection. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P308 + P311 : IF exposed or concerned: Call a POISON CENTER/doctor. |
| HS Number | 3822190090 |
References
- Cold Spring Harbor Laboratory Press, New York
E. Harlow, D. Lane. Antibodies, A Laboratory Manual, 1988, p404, p506, p594.
Documents
Safety Data Sheet (SDS)
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