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Published TCIMAIL newest issue No.201
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CAS RN: 95713-52-3 | Product Number: D2259
Nα-(5-Fluoro-2,4-dinitrophenyl)-L-alaninamide [HPLC Labeling Reagent for e.e. Determination]
![Thumbnail of Nalpha-(5-Fluoro-2,4-dinitrophenyl)-L-alaninamide [HPLC Labeling Reagent for e.e. Determination]](/medias/D2259.jpg?context=bWFzdGVyfHJvb3R8MTUxNDN8aW1hZ2UvanBlZ3xhRFUyTDJnME55ODRPVE0wTlRnd016VTFNVEF5TDBReU1qVTVMbXB3Wnd8NDAwMTFmNDRlYzQ5NzA3ZTU3ZWNjZmI5ZmMxYzBhZGU2YjY2MjhhN2M0NWFmNjNmYmI0M2VjYTJlOGQyZGFiZg "Nalpha-(5-Fluoro-2,4-dinitrophenyl)-L-alaninamide [HPLC Labeling Reagent for e.e. Determination]")
![Chemical Structure of Nalpha-(5-Fluoro-2,4-dinitrophenyl)-L-alaninamide [HPLC Labeling Reagent for e.e. Determination]](/medias/D2259.jpg?context=bWFzdGVyfHJvb3R8NDYxODZ8aW1hZ2UvanBlZ3xhR1UwTDJnek1TODRPVEl4TVRJM01USTFNREl5TDBReU1qVTVMbXB3Wnd8NjI1OWQxY2ZlY2U3YjA0OWM2YWZiNmIxNTBmYTlhNGZlOTgxYmE3MzkzNGE5NmY5YjA2MDdjOWFmODk1N2NlYw "Nalpha-(5-Fluoro-2,4-dinitrophenyl)-L-alaninamide [HPLC Labeling Reagent for e.e. Determination]")
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Purity: >98.0%(HPLC)
Synonyms:
- Nα-(2,4-Dinitro-5-fluorophenyl)-L-alaninamide
- Marfey's Reagent
- FDAA
Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
Shipping Information
|
|---|---|---|---|---|---|
| 100MG |
€75.00
|
6 | ≥100 |
|
|
| 1G |
€473.00
|
1 | ≥100 |
|
* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)
| Product Number | D2259 |
Purity / Analysis Method 
|
>98.0%(HPLC) |
| Molecular Formula / Molecular Weight | C__9H__9FN__4O__5 = 272.19 |
| Physical State (20 deg.C) | Solid |
| Storage Temperature
|
Room Temperature (Recommended in a cool and dark place, <15°C) |
| Packaging and Container
|
100MG-Glass Bottle with Plastic Insert (View image), 1G-Glass Bottle with Plastic Insert (View image) |
| CAS RN | 95713-52-3 |
| Reaxys Registry Number | 6820069 |
| PubChem Substance ID | 87568696 |
| MDL Number | MFCD00042049 |
Specifications
| Appearance | Light orange to Yellow to Green powder to crystal |
| Purity(HPLC) | min. 98.0 area% |
| Optical purity(LC) | min. 99.0 ee% |
| NMR | confirm to structure |
Properties (reference)
| Melting Point | 229 °C |
| Specific Rotation | 55° (C=1,Acetone) |
GHS
| Pictogram |
|
| Signal Word | Warning |
| Hazard Statements | H315 : Causes skin irritation. H319 : Causes serious eye irritation. |
| Precautionary Statements | P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ eye protection/ face protection. P302 + P352 : IF ON SKIN: Wash with plenty of water. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P362 + P364 : Take off contaminated clothing and wash it before reuse. P332 + P313 : If skin irritation occurs: Get medical advice/ attention. |
Related Laws:
Transport Information:
| HS Number | 2924297099 |
Application
Labeling Reagents for HPLC
Typical Procedure: Amino acid (2.5 µmol) is dissolved in 0.5 M NaHCO3 solution (100 µL) and 1% solution of Marfey’s reagent in acetone (200 µL) are mixed. The mixed solution is incubated at 40°C for 90 min and, after cooling, 2 M HCl (25 µL) is added. After 15~20-fold dilution in methanol or eluent, 10~20-µL aliquots are used for HPLC injection.
References
- P. Marfey, Carlsberg Res. Commun. 1984, 49, 591.
- G. Szokan, G. Mezo, F. Hudecz, J. Chromatogr. 1988, 444, 115.
- D. J. Aberhart, J.-A. Cotting, H.-J. Lin, Anal. Biochem. 1985, 151, 88.
- S. Kochhar, P. Christen, Anal. Biochem. 1989, 178, 17.
PubMed Literature
Product Documents (Note: Some products will not have analytical charts available.)
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