T-Fluor 480 Solution [for RNA staining]

×

Pureté:

Synonymes

Documents de produit:

SDS | Spécifications | CoA et autres Certificats | Graphiques analytiques

Informations générales | Spécifications et Propriétés | Sécurité et Réglementaire | Applications & Litterature

Informations supplémentaires sur le produit

Notes explicatives de cette page

Taille	Prix ​​unitaire	Belgique	Japon *	Quantité	Informations d'expédition
5ML	€80.00	Contactez-nous	32		Simulation d'expédition

*Stock disponible en Belgique : Expédition le jour même
*Stock disponible au Japon : Veuillez consulter la simulation d'expédition pour une estimation des délais d'expédition (hors articles réglementés et expédition avec Carboglace)

Ajouté à votre panier d'achat

Ajouter au panier

Devis en Vrac

Product Information

• Size : 5 mL
• Sterility : Filter-sterilized

Description

• Stains RNA in bacteria.
• T4436 contains T-Fluor 480 dissolved in PBS at a concentration of 800 µg/mL.
• Wavelengths of RNA stained with T-Fluor 480: λ_ex,max = 450 nm, λ_em,max = 485 nm.
• Very high fluorescence intensity. The signal can be observed without washing.
• Tested for RNA staining.

Directions for Use: RNA staining of E. coli with T-Fluor 480

• 1. Cultivate E. coli BW25113 on LB medium at 37 °C overnight.
  2. Add 1/100 volume of culture medium to LB medium with or without 100 µg/mL rifampicin (RNA synthesis inhibitor) and incubate at 37 °C for 4 hours.
  3. Dilute T4436 125-fold with PBS.
  4. After removing the supernatant by centrifugation, add the 125-fold diluted T-Fluor 480 solution to the pellet and stain at room temperature for 5 minutes.
  5. Observe under a fluorescence microscope using a filter for GFP.

Directions for Use: Observation of RNA in transparent biofilm

• 1. Incubate Methicillin-resistant Staphylococcus aureus (MRSA) in a glass-bottomed dish for 24 hours to form a biofilm.
  2. Remove the culture medium and suspended bacteria. Then fix and wash the biofilm.
  3. Dilute T4436 100-fold with PBS.
  4. Add the 100-fold diluted T-Fluor 480 solution to the dish and stain the biofilm at room temperature for 30 minutes.
  5. Add PBS or biofilm clearing reagent iCBiofilm-H1 (Product No. T4031) to the dish and observed the biofilm.

Storage

• This product is delivered frozen but can be stored at 4 °C. Please use it within six months after refrigeration.

Related Products

• T4031 Clearing Reagent iCBiofilm-H1 [for Biofilm]
• T4032 Clearing Reagent iCBiofilm-H2 [for Biofilm]
• I1275 Clearing Reagent iCBiofilm-B [for Biofilm]
• T4072 T-Fluor 480 [for RNA staining]
• R0079 Rifampicin
• A3144 Anti-αGal Polyclonal Antibody Biotin Conjugate
• A3226 Anti-GST Monoclonal Antibody Biotin Conjugate
• A3294 Anti-NeuGc Polyclonal Antibody Biotin Conjugate
• G0387 Goat Anti-Mouse IgG Biotin Conjugate
• G0389 Goat Anti-Rabbit IgG Biotin Conjugate
• G0432 Goat Anti-Mouse IgM Biotin Conjugate
• G0569 Goat Anti-Mouse IgG R-PE Conjugate
• G0577 Goat Anti-Rabbit IgG R-PE Conjugate
• H1619 Sheep Anti-Chicken IgY Biotin Conjugate
• S0951 Streptavidin from Streptomyces avidinii
• S0966 Streptavidin FITC Conjugate
• S0972 Streptavidin HRP Conjugate
• S0993 Streptavidin DTBTA-Eu³⁺ Conjugate
• T3531 Streptavidin Maleimide Conjugate