Published TCIMAIL newest issue No.199
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CAS RN: | Product Number: Y0021
E.coli / Yeast Protein Extraction Buffer
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Product Documents:
| Size | Unit Price | Belgium | Japan* | Quantity |
Shipping Information
|
|---|---|---|---|---|---|
| 100ML |
€ 86,00
|
1 | 7 |
|
* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)
Supplemental Product Information:
Product Information
- Volume : 100 mL
- Additives : WITHOUT protease inhibitors
Description
- This product is supplied as a ready-to-use solution to lyse cultured E. coli / yeast cells. Extracted protein can be used in downstream applications such as Western blotting and protein quantification. This product does not include protease inhibitors.
Storage
- Store at 4 °C.
Directions for Use: Lysis of E. coli cells
-
- Centrifuge E. coli cells at 5000 × g for 10 minutes.
- Remove as much medium as possible from pellet.
- Resuspend pellet in E.coli / Yeast Protein Extraction Buffer (Product No. Y0021). Use 5-10 mL buffer per gram pellet.
- Incubate for 10 minutes at room temperature.
- Centrifuge lysate at 15,000 × g for 10 minutes.
- Transfer the supernatant, containing extracted proteins, to a new tube for downstream applications.
Directions for Use: Lysis of yeast cells
-
- Centrifuge yeast cells at 3000 × g for 10 minutes.
- Remove as much medium as possible from pellet.
- Resuspend pellet in E.coli / Yeast Protein Extraction Buffer (Product No. Y0021). Use 2-4 mL buffer per gram pellet.
- Agitate at room temperature for 10 minutes.
- Centrifuge lysate at 14,000 × g for 10 minutes.
- Transfer the supernatant, containing extracted proteins, to a new tube for downstream applications.
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| Product Number | Y0021 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
| Appearance | Colorless to Almost colorlessclear liquid |
| Suitability for protein-analysis (E.coli) | to pass test |
| Suitability for protein-analysis (Yeast) | to pass test |
Properties (reference)
| Specific Gravity (20/20) | 1.06 |
GHS
Related Laws:
Transport Information:
| HS Number | 3822190090 |
Application
E.coli / Yeast Protein Extraction
References
- Non-detergent sulphobetaines: a new class of mild solubilization agents for protein purification
Product Articles
[Featured Products] Ready-to-use Solutions for Protein Extraction[TCIMAIL No.188] Ready-to-Use Solution for Protein Extraction from E. coli / Yeast Cells
Product Documents (Note: Some products will not have analytical charts available.)
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