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CAS RN: | Product Number: L0507
L-Lactate Assay Kit
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Product Documents:
| Size | Unit Price | Belgium | Japan* |
Shipping Information
|
|---|---|---|---|---|
| 1KIT |
€280.00
|
Contact Us | 24 |
* Stock available in Belgium: Shipment on the same day
* Stock available in Japan: Please check the Shipping Simulation for estimated shipments. (excludes regulated items and dry ice shipments)
Supplemental Product Information:
Product Information
- Components : Lactate Dye Mixture 1 vial, Lactate Enzyme Mixture 200 µL, Lactate Cofactor Mixture 500 µL, Lactate Assay Buffer 8 mL, Lactate Standard Solution 200 µL
- Size : 50 tests (96-well plate)
Description
- Lactate is a metabolite of glycolysis, the primary metabolic pathway in cells, and is a crucial indicator of cellular metabolic status. The quantification of lactate is widely utilised, particularly in cancer research and metabolic analysis. This kit enables straightforward quantification of L-lactic acid in both cell culture supernatants and intracellularly. Lactate concentration in samples can be accurately determined using a calibration curve created with the supplied lactate standard solution. The measurable lactate concentration range is 0.02-1.00 mM; samples exceeding 1.00 mM can be measured after dilution.
Storage
- Store at 4 °C. This product is photosensitive and should be protected from light. Use within 1 year.
Required Equipment
-
- Microplate reader (450 nm filter)
- 96-well microplate
- Incubator (37 °C)
- 0.1% Triton™-X (for intracellular lactate measurement)
- Ultrafiltration membrane filter (molecular weight cut-off: 10K) (for intracellular lactate measurement)
Step–by–Step Protocol
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| Product Number | L0507 |
| Physical State (20 deg.C) | Liquid |
Storage Temperature 
|
Refrigerated (0-10°C) |
| Condition to Avoid | Heat Sensitive |
Specifications
Properties (reference)
GHS
| Pictogram |
|
| Signal Word | Warning |
| Hazard Statements | H317 : May cause an allergic skin reaction. |
| Precautionary Statements | P501 : Dispose of contents/ container to an approved waste disposal plant. P272 : Contaminated work clothing should not be allowed out of the workplace. P261 : Avoid breathing mist or vapours. P280 : Wear protective gloves. P362 + P364 : Take off contaminated clothing and wash it before reuse. P333 + P313 : If skin irritation or rash occurs: Get medical advice/ attention. |
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