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CAS RN: 857447-79-1 | Product Number: B5440

Bis(tetrabutylammonium) Dihydrogen Pyrophosphate

Purity: >96.0%(T)
  • Bis(tetrabutylammonium) Dihydrogen Diphosphate
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Product Number B5440
Purity / Analysis Method >96.0%(T)
Molecular Formula / Molecular Weight C__3__2H__7__4N__2O__7P__2 = 660.90 
Physical State (20 deg.C) Solid
Storage Temperature <0°C
Store Under Inert Gas Store under inert gas
Condition to Avoid Air Sensitive,Hygroscopic,Heat Sensitive
CAS RN 857447-79-1
Reaxys Registry Number 9031313
PubChem Substance ID 354334284
Appearance White to Almost white powder to crystal
Purity(Nonaqueous Titration) min. 96.0 %
Properties (reference)
Pictogram Pictogram
Signal Word Warning
Hazard Statements H315 : Causes skin irritation.
H319 : Causes serious eye irritation.
Precautionary Statements P264 : Wash skin thoroughly after handling.
P280 : Wear protective gloves/ eye protection/ face protection.
P302 + P352 : IF ON SKIN: Wash with plenty of water.
P337 + P313 : If eye irritation persists: Get medical advice/ attention.
P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P362 + P364 : Take off contaminated clothing and wash it before reuse.
P332 + P313 : If skin irritation occurs: Get medical advice/ attention.
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Solid-phase Synthesis of DNA and RNA 5'-O-Triphosphates

After oligonucleotide synthesis:
5-Chloro-saligenyl-N,N-diisopropylphosphoramidite (Reagent I, C3326) is coupled to the 5'-end of the DMT-off oligonucleotide. Oxidation of the formed 5'-phosphite using the same oxidizing reagent used in standard oligonucleotide synthesis leads to support-bonded 5'-cycloSal-oligonucleotides. Reaction with pyrophosphate (Reagent II, B5440) yields the corresponding 5'-triphosphates. The 5'-triphosphorylated oligonucleotides are obtained after cleavage from the support in high purity and excellent yields. The whole reaction sequence was adapted to be used on a standard oligonucleotide synthesizer.


PubMed Literature

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