text.skipToContent text.skipToNavigation

Maximum quantity allowed is 999

Please select the quantity

CAS RN: | Product Number: H1746

Horseradish Peroxidase NHS Ester Conjugate (0.2mg×3)

  • Horseradish Peroxidase NHS Ester Activated (0.2mg×3)
  • HRP NHS (0.2mg×3)
37   Contact Us Contact Us
Available Stock: Prompt shipment (for products) in Saitama/Hyogo warehouse. Stock in other WH: 2-3 Business Days
* Please contact us if you need further information.
* To send your quote request for bulk quantities, please click on the "Request Bulk Quote" button. Please note that we cannot offer bulk quantities for some products.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.


Product Information

Volume/Quantity : 0.2 mg x 3 vial
Conjugation : Peroxidase from Horseradish (HRP) was conjugated to NHS Ester.
Product Form : Horseradish Peroxidase NHS Ester Conjugate. Lyophilized in 10 mM sodium citrate, 15 mM NaCl, 50 mM sucrose, pH 6.0.
Preservative : None


HRP NHS Ester Conjugate is designed to facilitate the conjugation of the HRP to proteins, peptides and ligands that contain an amino group (NH2). It can be used to label antibodies with a simple mixing.


Store at −20 °C to −80 °C (Preferably at −80 °C). Reconstitute this reagent immediately before use.
Do not prepare stock solutions for storage. Discard any unused reconstituted reagent.


  1. Dissolve the amine compound to be conjugated at 2 - 10 mg/mL in a buffer.
    Note: Small molecules containing azide or amino groups such as sodium azide need to be removed. Tris buffer and other amine containing buffers will also interfere with the labeling reaction.
    We recommend using the amine free buffer (e.g PBS, Phosphate buffer, Borate buffer, Bicarbonate buffer) pH range 7 - 9.
    The amount of antibody used for labeling ideally should correspond to molar ratios between 1:4 and 1:1 antibody to HRP. This vial contains 0.2 mg of HRP NHS and can effectively modify 0.2 - 0.8 mg of antibody.
  2. Equilibrate the vial to room temperature.
  3. Reconstitute the contents of the vial with 20 - 100 μL of a buffer to obtain a 2 - 10 mg/mL solution of HRP NHS.
    Note: Do not vortex or heat the HRP NHS.
  4. Immediately mix the amine-containing molecule and HRP NHS solutions and incubate for 2 hours at 37 °C.
    Note: Alternatively, the reaction may be initiated by adding amine-containing molecule solution to HRP-NHS vial.
  5. To quench the reaction, add 200 μL of 100 mM Tris-HCl Buffer pH 7.5 and incubate for 1 hour at 37 °C.
  6. If desired, desalt, dialyze or purify the conjugate.

Related Products

B5944 Bovine Serum Albumin Maleimide Conjugate (1mg×3)
T3531 Streptavidin Maleimide Conjugate
H1621 Horseradish Peroxidase Maleimide Conjugate (0.5mg×3)

Product Number H1746
Physical State (20 deg.C) Solid
Storage Temperature -80°C
Condition to Avoid Light Sensitive,Heat Sensitive
Appearance White to Brown powder to crystal
Titer min. 180 unit/mg
Functionality test to pass test (Amide-bond formation test with primary amine)
Properties (reference)
Related Laws:
Transport Information:
Documents (Note: Some products will not have analytical charts available.)
Safety Data Sheet (SDS)
Please select Language.

The requested SDS is not available.

Please Contact Us for more information.

C of A & Other Certificates
Please enter Lot Number Incorrect Lot Number is entered
Analytical Charts
Please enter Lot Number Incorrect Lot Number is entered

The requested analytical chart is not available. Sorry for the inconvenience.

Other Documents