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CAS RN: | Product Number: H1746
Horseradish Peroxidase NHS Ester Conjugate (0.2mg×3)
Purity:
Synonyms:
- Horseradish Peroxidase NHS Ester Activated (0.2mg×3)
- HRP NHS (0.2mg×3)
Product Documents:
Size | Unit Price | Saitama (Kawaguchi) | Hyogo (Amagasaki) | Stock in other WH |
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* The displayed price is the unit price and does not include consumption tax. The unit prices displayed are the latest and are subject to change without notice.
* To send your quote request for bulk quantities, please click on the "Request Bulk Quote" button. Please note that we cannot offer bulk quantities for some products.
*TCI frequently reviews storage conditions to optimize them. Please note that the latest information on the storage temperature for the products is described on our website.
Supplemental Product Information:
Product Information
Volume/Quantity : 0.2 mg x 3 vialConjugation : Peroxidase from Horseradish (HRP) was conjugated to NHS Ester.
Product Form : Horseradish Peroxidase NHS Ester Conjugate. Lyophilized in 10 mM sodium citrate, 15 mM NaCl, 50 mM sucrose, pH 6.0.
Preservative : None
Description
HRP NHS Ester Conjugate is designed to facilitate the conjugation of the HRP to proteins, peptides and ligands that contain an amino group (NH2). It can be used to label antibodies with a simple mixing.
Storage
Store at −20 °C to −80 °C (Preferably at −80 °C). Reconstitute this reagent immediately before use.
Do not prepare stock solutions for storage. Discard any unused reconstituted reagent.
Protocol
- Dissolve the amine compound to be conjugated at 2 - 10 mg/mL in a buffer.
Note: Small molecules containing azide or amino groups such as sodium azide need to be removed. Tris buffer and other amine containing buffers will also interfere with the labeling reaction.
We recommend using the amine free buffer (e.g PBS, Phosphate buffer, Borate buffer, Bicarbonate buffer) pH range 7 - 9.
The amount of antibody used for labeling ideally should correspond to molar ratios between 1:4 and 1:1 antibody to HRP. This vial contains 0.2 mg of HRP NHS and can effectively modify 0.2 - 0.8 mg of antibody. - Equilibrate the vial to room temperature.
- Reconstitute the contents of the vial with 20 - 100 μL of a buffer to obtain a 2 - 10 mg/mL solution of HRP NHS.
Note: Do not vortex or heat the HRP NHS. - Immediately mix the amine-containing molecule and HRP NHS solutions and incubate for 2 hours at 37 °C.
Note: Alternatively, the reaction may be initiated by adding amine-containing molecule solution to HRP-NHS vial. - To quench the reaction, add 200 μL of 100 mM Tris-HCl Buffer pH 7.5 and incubate for 1 hour at 37 °C.
- If desired, desalt, dialyze or purify the conjugate.
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Product Number | H1746 |
Physical State (20 deg.C) | Solid |
Storage Temperature ![]() |
Frozen (-80°C) |
Condition to Avoid | Light Sensitive,Heat Sensitive |
Specifications
Appearance | White to Brown powder to crystal |
Titer | min. 180 unit/mg |
Functionality test | to pass test (Amide-bond formation test with primary amine) |
Properties (reference)
GHS
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