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CAS RN: | Product Number: P3337

BPMP-4Thia Solution [for Biochemical Research]

Chemical Structure of BPMP-4Thia Solution  [for Biochemical Research]

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Supplemental Product Information:

Product Information

  • Volume : 0.1 mL (10 tests for mouse imaging)
  • Product Form : DMSO solution
  • Excitation / Emission : 610 nm / 661 nm (in Dibutyl Ether)

Description

  • BPMP-4Thia is a small compound that emits strong fluorescence in hydrophobic environment. It can dye clearly not only cellular lipid droplets but also tissue lipid droplets. It can be used in combination with multiple fluorescent dyes.
  • This product was developed by Dr. Toshitada Yoshihara.

Direction for Use

  • Lipid cell imaging

    1. Dilute BPMP-4Thia solution 5-fold with DMSO, then dilute it 2000-fold with medium such as DMEM.
    2. Remove culture medium and wash cells twice with PBS.
    3. Add BPMP-4Thia-containing medium to cells.
    4. Incubate cells at 37 °C for 30 minutes.
    5. Remove medium and wash cells twice with PBS.
    6. Observe stained lipid droplets.
  • Normal or fatty liver mouse imaging

    1. Dilute BPMP-4Thia solution 10-fold with saline containing 10% BSA.
    2. Inject the 100 µL of BPMP-4Thia stock solution into the tail vein of eight-week-old mouse under anesthesia 15 minutes before the fluorescence imaging experiment.
    3. Expose the liver of the mouse and observe it.

Storage

  • Store at −20 °C and protect from light. Avoid repeated freezing and thawing.

Related Products

  • D6131 DBC30 (2mg/mL in Dimethyl Sulfoxide) [for Biochemical Research]
  • P3152 PC6S (1mg/mL in Dimethyl Sulfoxide) [for Biochemical Research]
  • P3336 BPMP-2Thia (5mM in Dimethyl Sulfoxide) [for Biochemical Research]

Product Number P3337
Grade SU
Molecular Formula / Molecular Weight C__3__2H__2__5BF__2N__6S__4 = 670.64 
Physical State (20 deg.C) Liquid
Storage Temperature Frozen (-20°C)
Store Under Inert Gas Store under inert gas
Condition to Avoid Light Sensitive,Air Sensitive,Heat Sensitive
Specifications
Appearance Dark green to Dark blue to Black clear liquid
Cell staining test to pass test
Properties (reference)
GHS
Signal Word Warning
Hazard Statements H227 : Combustible liquid.
Precautionary Statements P501 : Dispose of contents/ container to an approved waste disposal plant.
P210 : Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P280 : Wear protective gloves/ eye protection/ face protection.
P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
P403 : Store in a well-ventilated place.
Related Laws:
Fire Defense Law Group-4-3-III
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