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CAS RN: 541520-65-4 | Product Number: P3419

Protein Gel Stain Tangerine (in Dimethyl Sulfoxide) [for Electrophoresis]

Chemical Structure of Protein Gel Stain Tangerine (in Dimethyl Sulfoxide) [for Electrophoresis]
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× Protein Gel Stain Tangerine (in Dimethyl Sulfoxide) [for Electrophoresis]

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Synonyms:
  • (E)-4-[4-[2-(9-Ethyl-9H-carbazol-3-yl)vinyl]quinolin-1-ium-1-yl]butane-1-sulfonate (in Dimethyl Sulfoxide)
Product Documents:
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Supplemental Product Information

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0.25ML
¥11,000
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Supplemental Product Information:

Product Information

  • Volume : 250 µL (5000X concentrated solution in DMSO)

Description

  • Protein Gel Stain Tangerine is a fluorescent dye used to stain proteins in gels following SDS-PAGE. Its straightforward staining procedure enables the detection of proteins at concentrations as low as 5-10 ng. This product is fully miscible with water-based solvents, bypassing the use of harsh organic solvents to make it safe for use. When stained in the presence of salts such as 150 mM NaCl, proteins are not fixed in the gel and can be transferred to a blotting membrane. This enables downstream applications such as Western blotting. If protein fixation is to be carried out, this product should first be dissolved in a solution such as 7.5% (v/v) acetic acid. This product should not be used in solutions containing methanol, as methanol removes the SDS coating from proteins, markedly reducing the staining signal. After staining is completed, simply rinse the gel twice with water before imaging. This product exhibits excitation maxima at 290 nm and 490 nm and an emission maximum at 650 nm. Detection can be performed using a UV transillumination system or Imager. Please note that this product is not suitable for staining two-dimensional (2D) gels or isoelectric focusing (IEF) gels.

Storage

  • Store at 4 °C. This product is photosensitive and should be protected from light. Use within 6 months.

Step–by–Step Protocol

Related Products

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  • C3488 Coomassie Brilliant Blue G-250 (Ready-to-use solution) [for Electrophoresis]
  • B3193 Coomassie Brilliant Blue G-250 [for Electrophoresis]
  • B3194 Coomassie Brilliant Blue R-250 [for Electrophoresis]

Product Number P3419
Grade SU
Molecular Formula / Molecular Weight C__2__9H__2__8N__2O__3S = 484.61 
Physical State (20 deg.C) Liquid
Storage Temperature Refrigerated (0-10°C)
Condition to Avoid Light Sensitive,Heat Sensitive
CAS RN 541520-65-4
Specifications
Appearance Red to Dark red to Brown clear liquid
Gel fluorescence staining test to pass test
Absorbance measurement test to pass test
Properties (reference)
Specific Gravity (20/20) 1.09
GHS
Signal Word Warning
Hazard Statements H227 : Combustible liquid.
Precautionary Statements P501 : Dispose of contents/ container to an approved waste disposal plant.
P210 : Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P280 : Wear protective gloves/ eye protection/ face protection.
P370 + P378 : In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
P403 : Store in a well-ventilated place.
Related Laws:
Fire Defense Law Group-4-3-III
Transport Information:
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Safety Data Sheet (SDS)
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