CAS RN: | Product Number: R0229
Recombinant Griffithsia sp. lectin (= rGRFT) expressed in Escherichia coli
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Avoid multiple freeze-thaw cycles. Add some preservative, e.g. sodium azide upon opening for the first time, and store at 2-10 °C.
Product code: R0229
Product name: Recombinant Griffithsia sp. lectin (= rGRFT) expressed in Escherichia coli
Quantity: 1 ml per vial (1 mg/1 ml: Lowry method)
Product form: Purified recombinant GRFT, Liquid
Buffer: Phosphate buffered saline [PBS] (-), pH7.4
Molecular Mass: 13 kDa
Preservative : None
The rGRFT is a carbohydrate-binding protein (Lectin) derived from Griffithsia sp. The rGRFT gene product is expressed in Escherichia coli. This lectin preferably binds to a [Manα(1-2)Man] glycan epitope. Consequently, rGRFT exhibits a hemagglutination activity to a guinea pig (but not rabbit) reticulocyte through the glycan binding to subsites and subunits of the lectin.
Store at -20°C to -80°C. Avoid repetitive freezing and thawing. After thawing the product, it's recommended to store at 2-10°C with an additional preservative (e.g., 0.02-0.05% [w/v] sodium azide).
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L0169 Lectin, Fucose specific (= AOL) from Aspergillus oryzae (5mg/mL, PBS pH6.5)
|Physical State (20 deg.C)||Liquid|
|Condition to Avoid||Heat Sensitive|
|Appearance||Colorless to Light yellow to Light orange clear liquid|
|Concentration(Lowry method)||0.9 to 1.5 mg/mL|
|Molecular weight||10000 to 25000(SDS-PAGE)|
|Hemagglutination activity||max. 16 micro g/mL(pH7.2)|
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