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CAS RN: 62229-50-9 | Product Number: R0262
rhEGF (Human, Recombinant) [Optimized for Cell Culture] (100µg/vial)
Purity:
- Epidermal Growth Factor (Human, Recombinant) (100µg/vial)
- EGF (Human, Recombinant) (Human, Recombinant) (100µg/vial)
| Size | Unit Price | Saitama (Kawaguchi) | Hyogo (Amagasaki) | Stock in other WH |
Shipping Information
|
|---|---|---|---|---|---|
| 1VIAL |
¥23,000
|
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Supplemental Product Information:
Description
This product contains 100 µg of recombinant human EGF produced in E. Coli, lyophilized from a 1mg/mL solution containing 1x PBS. Recombinant human EGF comprises a 53 amino acid-length, non-glycosylated polypeptide chain, with a molecular mass of 6.2kDa. Apart from being a potent mitogenic factor, EGF stimulates the proliferation of a wide variety of epithelial cells, and is a common medium additive in both 2D and 3D cell culture.
Specifications
Biological Activity (Balb/c Proliferation Assay) : ED50 < 0.1 ng/mL (> 1 x 107 IU/mL)Purity (SDS/PAGE) : >95.0%
Mycoplasma Contamination (PCR) : No bands corresponding to Mycoplasma detected
Endotoxin Level (Chromogenic LAL Assay) : < 1.0 EU/µg
Amino Acid Sequence
NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
Storage
Store at -80°C. Post-reconstitution, the addition of a carrier protein such as 0.1% BSA is recommended for improved stability. Avoid multiple freeze-thaw cycles.
| Product Number | R0262 |
| Physical State (20 deg.C) | Solid |
Storage Temperature
|
Frozen (-80°C) |
| Condition to Avoid | Heat Sensitive |
| CAS RN | 62229-50-9 |
| Appearance | White to Almost white powder to crystal |
| Biological Activity (Balb/c-3T3 proliferation assay) | ED50 ≤ 0.1ng/mL |
| Endotoxin LevelEndotoxin Level (chromogenic LAL assay) | max. 1.0 EU/µg |
| Mycoplasma Contamination (PCR) | No bands corresponding to mycoplasma were detected |
| Purity (SDS-PAGE/CBB) | min. 95.0 % |
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Safety Data Sheet (SDS)
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Specifications
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C of A & Other Certificates
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Sample C of A
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Analytical Charts
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