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CAS RN: 106096-93-9 | Product Number: R0263
rhFGF2 (Human, Recombinant) [Optimized for Cell Culture] (50µg/vial)
Purity:
- Recombinant Human Basic Fibroblast Growth Factor (Human, Recombinant) (50µg/vial)
- FGF2 (Human, Recombinant) (Human, Recombinant) (50µg/vial)
- Fibroblast Growth Factor 2 (Human, Recombinant) (Human, Recombinant) (50µg/vial)
- Basic Fibroblast Growth Factor (Human, Recombinant) (Human, Recombinant) (50µg/vial)
- rhbFGF, Recombinant Human Fibroblast Growth Factor 2 (Human, Recombinant) (50µg/vial)
| Size | Unit Price | Saitama (Kawaguchi) | Hyogo (Amagasaki) | Stock in other WH |
Shipping Information
|
|---|---|---|---|---|---|
| 1VIAL |
¥30,000
|
14 | It can be shipped from Saitama warehouse on the same day* | Contact Us |
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Supplemental Product Information:
Description
This product contains 50 µg of recombinant human FGF2 produced in E. Coli, lyophilized from a 1mg/mL solution containing 20mM Tris-HCl (pH 7.4) and 1M NaCl. Recombinant human FGF2 comprises a 154 amino acid-length, non-glycosylated polypeptide chain, with a molecular mass of 17.2kDa. FGF2 is a common medium additive in both 2D and 3D cell culture; aside from being a potent mitogenic factor, FGF2 is able to stimulate the proliferation of a wide variety of mesothelial and neuroectodermal cells both in vitro and in vivo.
Specifications
Biological Activity (Balb/c Proliferation Assay) : ED50 < 0.1 ng/mL (> 1 x 107 IU/mL)
Purity (SDS/PAGE) : >95.0%
Mycoplasma Contamination (PCR) : No bands corresponding to Mycoplasma detected
Endotoxin Level (Chromogenic LAL Assay) : < 1.0 EU/µg
Amino Acid Sequence
AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS
Storage
Store at -80°C. Post-reconstitution, the addition of a carrier protein such as 0.1% BSA is recommended for improved stability. Avoid multiple freeze-thaw cycles.
| Product Number | R0263 |
| Physical State (20 deg.C) | Solid |
Storage Temperature
|
Frozen (-80°C) |
| Condition to Avoid | Heat Sensitive |
| CAS RN | 106096-93-9 |
| Appearance | White to Almost white powder to crystal |
| Biological Activity (Balb/c-3T3 proliferation assay) | ED50 ≤ 0.1ng/mL |
| Endotoxin LevelEndotoxin Level (chromogenic LAL assay) | max. 1.0 EU/µg |
| Mycoplasma Contamination (PCR) | No bands corresponding to mycoplasma were detected |
| Purity (SDS-PAGE/CBB) | min. 95.0 % |
| Pictogram |
|
| Signal Word | Warning |
| Hazard Statements | H315 : Causes skin irritation. H319 : Causes serious eye irritation. |
| Precautionary Statements | P264 : Wash skin thoroughly after handling. P280 : Wear protective gloves/ eye protection/ face protection. P302 + P352 : IF ON SKIN: Wash with plenty of water. P337 + P313 : If eye irritation persists: Get medical advice/ attention. P305 + P351 + P338 : IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P362 + P364 : Take off contaminated clothing and wash it before reuse. P332 + P313 : If skin irritation occurs: Get medical advice/ attention. |
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